Endoglin ELISA Kit

Catalog No. ABIN2042388

This Human Endoglin ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human Endoglin.

Quick Overview for Endoglin ELISA Kit (ABIN2042388)

Target: Endoglin (ENG)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 6-10000 pg/mL

Application: ELISA

Product Details Endoglin ELISA Kit

Minimum Detection Limit: 6 pg/mL

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 15 pg/mL

Components: 96-well plate pre-coated with anti-CD105, Human
Lyophilized recombinant CD105, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Application Details

Plate: Pre-coated

Protocol: Sandwich ELISA quantitative detection kit for Human CD105 in cell culture supernates, serum and plasma (heparin, EDTA).CD105, also called Endoglin, is a homodimeric membrane glycoprotein primarily associated with human vascular endothelium. It is also found on bone marrow proerythroblasts, activated monocytes, and lymphoblasts in childhood leukemia. Endoglin is a component of the transforming growth factor-beta (TGFB) receptor complex and binds TGFB1 with high affinity.1 CD105 gene is mapped to 9q34.1. The coding region of the gene contains 14 exons.2 The protein consists of a homodimer of 180kDA with disulfide links. Endoglin has a role in the development of the cardiovascular system and in vascular remodeling. Its expression is regulated during heart development. Furthermore, it also has a role in the balance of ALK1 and ALK5 signaling to regulate endothelial cell proliferation.3 Moreover, the elevated expression of endoglin in the surgically excised CNVMs suggested a persisting postmitotic activation in an advanced stage of neovascular tissue.4 The standard product used in this kit is extracellular part of recombinant human CD105, from E26 to G586. As a result of glycosylation, the molecular mass is 75-85kD.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for Endoglin

Target: Endoglin (ENG)

Alternative Name: CD105

Gene ID: 2022

UniProt: P17813