Endoglin ELISA Kit

Catalog No. ABIN6720098

Product Details Endoglin ELISA Kit

Target: Endoglin (ENG)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156 pg/mL - 10000 pg/mL

Minimum Detection Limit: 156 pg/mL

Application: ELISA

Purpose: Sandwich ELISA for Quantitative Detection of Antigen

Sample Type: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Characteristics:

Synonyms: AI528660, AI662476, CD 105, CD105 antigen, Cell surface MJ7/18 antigen, END, Endoglin, ENG, FLJ41744, HHT1, ORW, ORW1, Osler Rendu Weber syndrome 1, OTTMUSP00000012838, OTTMUSP00000012839, RP11 228B15.2, RP23 17P12.4, S endoglin, Transmembrane glycoprotein

Background: CD105, also called Endoglin, is a homodimeric membrane glycoprotein primarily associated with human vascular endothelium. It is also found on bone marrow proerythroblasts, activated monocytes, and lymphoblasts in childhood leukemia. Endoglin is a component of the transforming growth factor-beta(TGFB) receptor complex and binds TGFB1 with high affinity.1 CD105 gene is mapped to 9q34.1. The coding region of the gene contains 14 exons.2 The protein consists of a homodimer of 180 kDA with disulfide links. Endoglin has a role in the development of the cardiovascular system and in vascular remodeling. Its expression is regulated during heart development. Furthermore, it also has a role in the balance of ALK1 and ALK5 signaling to regulate endothelial cell proliferation.3 Moreover, the elevated expression of endoglin in the surgically excised CNVMs suggested a persisting postmitotic activation in an advanced stage of neovascular tissue.4 The standard product used in this kit is extracellular part of recombinant huma...

Gene Name: ENG

Production: Natural and recombinant human CD105. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: CHO, Immunogen sequence: E26-G586

Application Details

Application Notes:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human CD105 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human CD105 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 156pg/mL-10000pg/mL

Sample Volume: 100 μL

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT,4 °C,-20 °C

Storage Comment: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Target Details for Endoglin

Target: Endoglin (ENG)

Alternative Name: CD105 (ENG Products)

Synonyms: ENG ELISA Kit, MGC137842 ELISA Kit, DKFZp469D0419 ELISA Kit, END ELISA Kit, HHT1 ELISA Kit, ORW1 ELISA Kit, AI528660 ELISA Kit, AI662476 ELISA Kit, CD105 ELISA Kit, S-endoglin ELISA Kit, endoglin ELISA Kit, ENG ELISA Kit, Eng ELISA Kit

Gene ID: 2022

NCBI Accession: NP_000109

UniProt: P17813