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Clusterin ELISA Kit

Human Clusterin ELISA Kit Colorimetric assay for quantification of Human Clusterin.
Catalog No. ABIN2042407

Quick Overview for Clusterin ELISA Kit (ABIN2042407)

Target

See all Clusterin (CLU) ELISA Kits
Clusterin (CLU)

Reactivity

  • 10
  • 7
  • 7
  • 4
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
Human

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

0.8-50 ng/mL

Application

ELISA
  • Minimum Detection Limit

    0.8 ng/mL

    Cross-Reactivity (Details)

    There is no detectable cross-reactivity with other relevant proteins.

    Sensitivity

    < 20 pg/mL

    Components

    96-well plate pre-coated with anti-Clusterin, Human
    Lyophilized recombinant Clusterin, Human standard, 10 ng/tube, 2Ea
    Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
    Sample diluent buffer, 30 mL
    Antibody diluent buffer, 10 mL
    ABC diluent buffer, 10 mL
    TMB color developing agent, 10 mL
    TMB stop solution, 10 mL
  • Plate

    Pre-coated

    Protocol

    Sandwich ELISA quantitative detection kit for Human Clusterin in cell culture supernates, serum, plasma (heparin, EDTA), saliva and urine.Clusterin (apolipoprotein J) is a 75 - 80kD disulfide-linked heterodimeric protein associated with the clearance of cellular debris and apoptosis.[1] In humans, clusterin is encoded by the CLU gene.This protein has several synonyms dimeric acidic glycoprotein (DAG protein), testosterone repressed prostate message-2 (TRPM-2), sulfated glycoprotein-2 (SGP-2) and complement lysis inhibitor (CLI). Clusterin was mapped to mouse chromosome 14. Clusterin is a ubiquitously expressed molecule thought to influence a variety of processes including cell death. In the brain, it accumulates in dying neurons following seizures and hypoxic-ischemic (H-I) injury. clusterin may be a new therapeutic target to modulate non-caspase-dependent neuronal death following acute brain injury.

    Restrictions

    For Research Use only
  • Storage

    4 °C/-20 °C
  • Target See all Clusterin (CLU) ELISA Kits

    Clusterin (CLU)

    Alternative Name

    Clusterin

    Gene ID

    1191

    UniProt

    P10909

    Pathways

    Apoptosis, Negative Regulation of intrinsic apoptotic Signaling
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