CNTF ELISA Kit
Quick Overview for CNTF ELISA Kit (ABIN2042409)
Target
See all CNTF ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
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Minimum Detection Limit
- 31.2 pg/mL
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Cross-Reactivity (Details)
- There is no detectable cross-reactivity with other relevant proteins.
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Sensitivity
- < 10 pg/mL
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Components
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96-well plate pre-coated with anti-CNTF, Rat
Lyophilized recombinant CNTF, Rat standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
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Ciliary Neurotrophic Factor (CNTF) ELISA Kit
CNTF Reactivity: Mouse Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Ciliary Neurotrophic Factor (CNTF) ELISA KitCNTF Reactivity: Human Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Ciliary Neurotrophic Factor (CNTF) ELISA KitCNTF Reactivity: Rabbit Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Ciliary Neurotrophic Factor (CNTF) ELISA KitCNTF Reactivity: Rat Colorimetric Sandwich ELISA 39 pg/mL - 2500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Ciliary Neurotrophic Factor (CNTF) ELISA KitCNTF Reactivity: Chicken Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Ciliary Neurotrophic Factor (CNTF) ELISA KitCNTF Reactivity: Pig Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Ciliary Neurotrophic Factor (CNTF) ELISA KitCNTF Reactivity: Rat AA 2-200 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Ciliary Neurotrophic Factor (CNTF) ELISA KitCNTF Reactivity: Human Colorimetric Sandwich ELISA 47-3000 pg/mL Cell Lysate, Plasma, Serum
Ciliary Neurotrophic Factor (CNTF) ELISA KitCNTF Reactivity: Rat Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
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Plate
- Pre-coated
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Protocol
- Sandwich ELISA quantitative detection kit for Rat CNTF in cell culture supernates, serum and plasma (heparin, EDTA, citrate.Ciliary neurotrophic factor (CNTF) is a potent polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes survival, neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The hCNTF gene is located on chromosome 11, as determined using human-hamster somatic cell hybrids. The CNTF protein is highly conserved in evolution. The amino acid (aa) sequences of rat and rabbit CNTF translated from cDNAs display approx. 85% homology with the deduced aa sequence encoding hCNTF. CNTF induces weight loss and improves glucose tolerance in humans and rodents. CNTF is thought to act centrally by inducing hypothalamic neurogenesis to modulate food intake and peripherally by altering hepatic gene expression, in a manner similar to that of leptin.
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Restrictions
- For Research Use only
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Storage
- 4 °C/-20 °C
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- CNTF (Ciliary Neurotrophic Factor (CNTF))
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Alternative Name
- CNTF
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Gene ID
- 25707
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UniProt
- P20294
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Pathways
- JAK-STAT Signaling
Target See all CNTF ELISA Kits
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