C5A ELISA Kit

Catalog No. ABIN2042411

This Colorimetric ELISA kit is designed for the quantitative measurement of Human C5A.

Quick Overview for C5A ELISA Kit (ABIN2042411)

Target: C5A (C5a) (Complement Component 5a (C5a))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2-2000 pg/mL

Application: ELISA

Product Details C5A ELISA Kit

Minimum Detection Limit: 31.2 pg/mL

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components: 96-well plate pre-coated with anti-Complement C5a, Human
Lyophilized recombinant Complement C5a, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Application Details

Plate: Pre-coated

Protocol: Sandwich ELISA quantitative detection kit for Human complement C5a in cell culture supernates, serum and plasma (heparin, EDTA).C5a is a protein fragment released from complement component C5. This gene is mapped to 9q33.2. The protein encoded by this gene is the fifth component of complement, which plays an important role in inflammatory and cell killing processes. This protein is comprised of alpha and beta polypeptide chains that are linked by a disulfide bridge. An activation peptide, C5a, which is an anaphylatoxin that possesses potent spasmogenic and chemotactic activity, is derived from the alpha polypeptide via cleavage with a convertase. The C5b macromolecular cleavage product can form a complex with the C6 complement component, and this complex is the basis for formation of the membrane attack complex, which includes additional complement components. Mutations in this gene cause complement component 5 deficiency, a disease where patients show a propensity for severe recurrent infections. Defects in this gene have also been linked to susceptibility to liver fibrosis and to rheumatoid arthritis.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for C5A

Target: C5A (C5a) (Complement Component 5a (C5a))

Alternative Name: Complement C5a

Gene ID: 727

UniProt: P01031

Pathways: Complement System, Carbohydrate Homeostasis