C5A ELISA Kit
Quick Overview for C5A ELISA Kit (ABIN2042412)
Target
See all C5A (C5a) ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
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Minimum Detection Limit
- 15.6 pg/mL
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Cross-Reactivity (Details)
- There is no detectable cross-reactivity with other relevant proteins.
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Sensitivity
- < 10 pg/mL
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Components
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96-well plate pre-coated with anti-Complement C5a, Mouse
Lyophilized recombinant Complement C5a, Mouse standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
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Complement Component 5a (C5a) ELISA Kit
C5a Reactivity: Human Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Complement Component 5a (C5a) ELISA KitC5a Reactivity: Mouse Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Complement Component 5a (C5a) ELISA KitC5a Reactivity: Rat Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum
Complement Component 5a (C5a) ELISA KitC5a Reactivity: Rat Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Complement Component 5a (C5a) ELISA KitC5a Reactivity: Rat Colorimetric Sandwich ELISA 15.63 ng/mL - 1000 ng/mL Cell Culture Supernatant, Plasma, Serum
Complement Component 5a (C5a) ELISA KitC5a Reactivity: Monkey Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Complement Component 5a (C5a) ELISA KitC5a Reactivity: Rabbit Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Complement Component 5a (C5a) ELISA KitHigh Sensitivity C5a Reactivity: Mouse Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Complement Component 5a (C5a) ELISA KitC5a Reactivity: Monkey Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Complement Component 5a (C5a) ELISA KitC5a Reactivity: Rabbit Colorimetric Sandwich ELISA 6.25 pg/mL - 400 pg/mL Plasma, Serum
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Plate
- Pre-coated
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Protocol
- Sandwich ELISA quantitative detection kit for Mouse Complement C5a in cell culture supernates, serum and plasma (heparin, EDTA).C5a is a protein fragment released from complement component C5. This gene is mapped to 9q33.2. The protein encoded by this gene is the fifth component of complement, which plays an important role in inflammatory and cell killing processes. This protein is comprised of alpha and beta polypeptide chains that are linked by a disulfide bridge. An activation peptide, C5a, which is an anaphylatoxin that possesses potent spasmogenic and chemotactic activity, is derived from the alpha polypeptide via cleavage with a convertase. The C5b macromolecular cleavage product can form a complex with the C6 complement component, and this complex is the basis for formation of the membrane attack complex, which includes additional complement components. Mutations in this gene cause complement component 5 deficiency, a disease where patients show a propensity for severe recurrent infections. Defects in this gene have also been linked to susceptibility to liver fibrosis and to rheumatoid arthritis.
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Restrictions
- For Research Use only
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Storage
- 4 °C/-20 °C
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- C5A (C5a) (Complement Component 5a (C5a))
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Alternative Name
- Complement C5a
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Gene ID
- 15139
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UniProt
- P06684
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Pathways
- Complement System, Carbohydrate Homeostasis
Target See all C5A (C5a) ELISA Kits
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