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EGF ELISA Kit

This Rat EGF ELISA Kit is a Colorimetric ELISA Kit designed to quantify Rat EGF.
Catalog No. ABIN2042445

Quick Overview for EGF ELISA Kit (ABIN2042445)

Target

See all EGF ELISA Kits
EGF (Epidermal Growth Factor (EGF))

Reactivity

  • 19
  • 14
  • 10
  • 5
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
Rat

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

7.8-500 pg/mL

Application

ELISA
  • Minimum Detection Limit

    7.8 pg/mL

    Cross-Reactivity (Details)

    There is no detectable cross-reactivity with other relevant proteins.

    Sensitivity

    < 1 pg/mL

    Components

    96-well plate pre-coated with anti-EGF, Rat
    Lyophilized recombinant EGF, Rat standard, 10 ng/tube, 2Ea
    Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
    Sample diluent buffer, 30 mL
    Antibody diluent buffer, 10 mL
    ABC diluent buffer, 10 mL
    TMB color developing agent, 10 mL
    TMB stop solution, 10 mL
  • Plate

    Pre-coated

    Protocol

    Sandwich ELISA quantitative detection kit for Rat EGF in cell culture supernates, serum, plasma (heparin, EDTA), tissue homogenates and urine.Epidermal growth factor (EGF) is a growth factor that plays an important role in the regulation of cell growth, proliferation and differentiation by binding to its receptor EGFR. EGF locus is mapped to 4q21-4qter. Human EGF is a 6045-Da protein with 53 amino acid residues and three intramolecular disulfide bones. EGF results in cellular proliferation, differentiation, and survival while plays a physiological role in the maintenance of oro-esophageal and gastric tissue integrity.

    Restrictions

    For Research Use only
  • Storage

    4 °C/-20 °C
  • Target See all EGF ELISA Kits

    EGF (Epidermal Growth Factor (EGF))

    Alternative Name

    EGF

    Gene ID

    25313

    UniProt

    P07522

    Pathways

    NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation
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