FURIN ELISA Kit

Catalog No. ABIN2042473

Human FURIN ELISA Kit Colorimetric assay for quantification of Human FURIN.

Quick Overview for FURIN ELISA Kit (ABIN2042473)

Target: FURIN (Furin (Paired Basic Amino Acid Cleaving Enzyme) (FURIN))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 2-20000 pg/mL

Application: ELISA

Product Details FURIN ELISA Kit

Minimum Detection Limit: 2 pg/mL

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components: 96-well plate pre-coated with anti-Furin, Human
Lyophilized recombinant Furin, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Application Details

Plate: Pre-coated

Protocol: Sandwich ELISA quantitative detection kit for Human Furin in cell culture supernates, tissue homogenates and cell lysates.Furin(Fur), also known as PACE, is a protein that in humans is encoded by the FURIN gene. The protein encoded by this gene is an enzyme which belongs to the subtilisin-like proprotein convertase family. The FUR gene is located approximately 1 kb upstream of the FES gene on chromosome 15q25-q26. This gene is thought to play a role in tumor progression. Furin is one of the proteases responsible for the proteolytic cleavage of HIV envelope polyprotein precursor gp160 to gp120 and gp41 prior to viral assembly. Furin is enriched in the Golgi apparatus, where it functions to cleave other proteins into their mature/active forms. Expression of furin in T-cells is required for maintenance of peripheral immune tolerance

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for FURIN

Target: FURIN (Furin (Paired Basic Amino Acid Cleaving Enzyme) (FURIN))

Alternative Name: Furin

Gene ID: 5045

UniProt: P09958

Pathways: Notch Signaling, Neurotrophin Signaling Pathway