LGALS1/Galectin 1 ELISA Kit
Quick Overview for LGALS1/Galectin 1 ELISA Kit (ABIN2042476)
Target
See all LGALS1/Galectin 1 (LGALS1) ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
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Minimum Detection Limit
- 6 pg/mL
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Cross-Reactivity (Details)
- There is no detectable cross-reactivity with other relevant proteins.
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Sensitivity
- < 5 pg/mL
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Components
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96-well plate pre-coated with anti-Galectin-1, Mouse
Lyophilized recombinant Galectin-1, Mouse standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
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Plate
- Pre-coated
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Protocol
- Sandwich ELISA quantitative detection kit for Mouse GALECTIN-1 in cell culture supernates, serum and plasma (heparin, EDTA).Galectin-1 is a protein that in humans is encoded by the LGALS1 gene.The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. LGALS1 may act as an autocrine negative growth factor that regulates cell proliferation.Baldini et al. stated that the mouse beta-galactoside-binding protein is an autocrine regulator of cell proliferation with a role in the maintenance of G0 and in the control of G2 traverse.[4] They found that galectin-1 was expressed in a subset of slowly dividing subventricular zone astrocytes, which included the neural stem cells. Intraventricular infusion experiments and phenotypic analysis of knockout mice showed that galectin-1 was an endogenous factor that promoted the proliferation of neural stem cells in adult mouse brain. The standard product used in this kit is recombinant mouse GALECTIN-1, Ala2-Glu135, with the molecular mass of 15kD.
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Restrictions
- For Research Use only
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Storage
- 4 °C/-20 °C
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- LGALS1/Galectin 1 (LGALS1) (Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))
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Alternative Name
- Galectin-1
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Gene ID
- 16852
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UniProt
- P16045
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Pathways
- Carbohydrate Homeostasis
Target See all LGALS1/Galectin 1 (LGALS1) ELISA Kits
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