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LGALS1/Galectin 1 ELISA Kit

Mouse LGALS1/Galectin 1 ELISA Kit Colorimetric assay for quantification of Mouse LGALS1/Galectin 1.
Catalog No. ABIN2042476

Quick Overview for LGALS1/Galectin 1 ELISA Kit (ABIN2042476)

Target

See all LGALS1/Galectin 1 (LGALS1) ELISA Kits
LGALS1/Galectin 1 (LGALS1) (Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))

Reactivity

  • 9
  • 8
  • 5
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Mouse

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

6-10000 pg/mL

Application

ELISA
  • Minimum Detection Limit

    6 pg/mL

    Cross-Reactivity (Details)

    There is no detectable cross-reactivity with other relevant proteins.

    Sensitivity

    < 5 pg/mL

    Components

    96-well plate pre-coated with anti-Galectin-1, Mouse
    Lyophilized recombinant Galectin-1, Mouse standard, 10 ng/tube, 2Ea
    Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
    Sample diluent buffer, 30 mL
    Antibody diluent buffer, 10 mL
    ABC diluent buffer, 10 mL
    TMB color developing agent, 10 mL
    TMB stop solution, 10 mL
  • Plate

    Pre-coated

    Protocol

    Sandwich ELISA quantitative detection kit for Mouse GALECTIN-1 in cell culture supernates, serum and plasma (heparin, EDTA).Galectin-1 is a protein that in humans is encoded by the LGALS1 gene.The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. LGALS1 may act as an autocrine negative growth factor that regulates cell proliferation.Baldini et al. stated that the mouse beta-galactoside-binding protein is an autocrine regulator of cell proliferation with a role in the maintenance of G0 and in the control of G2 traverse.[4] They found that galectin-1 was expressed in a subset of slowly dividing subventricular zone astrocytes, which included the neural stem cells. Intraventricular infusion experiments and phenotypic analysis of knockout mice showed that galectin-1 was an endogenous factor that promoted the proliferation of neural stem cells in adult mouse brain. The standard product used in this kit is recombinant mouse GALECTIN-1, Ala2-Glu135, with the molecular mass of 15kD.

    Restrictions

    For Research Use only
  • Storage

    4 °C/-20 °C
  • Target See all LGALS1/Galectin 1 (LGALS1) ELISA Kits

    LGALS1/Galectin 1 (LGALS1) (Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))

    Alternative Name

    Galectin-1

    Gene ID

    16852

    UniProt

    P16045

    Pathways

    Carbohydrate Homeostasis
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