GM-CSF ELISA Kit

Catalog No. ABIN2042486

This Colorimetric ELISA kit is designed for the quantitative measurement of Rat GM-CSF.

Quick Overview for GM-CSF ELISA Kit (ABIN2042486)

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6-1000 pg/mL

Application: ELISA

Product Details GM-CSF ELISA Kit

Minimum Detection Limit: 15.6 pg/mL

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components: 96-well plate pre-coated with anti-GM-CSF, Rat
Lyophilized recombinant GM-CSF, Rat standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Application Details

Plate: Pre-coated

Protocol: Sandwich ELISA quantitative detection kit for Rat GM-CSF in cell culture supernates, serum and plasma (heparin, EDTA).Granulocyte-macrophage colony-stimulating factor (GM-CSF) is also symbolized CSF2. Human GM-CSF is a glycoprotein that is essential for the in vitro proliferation and differentiation of precursor cells into mature granulocytes and macrophages. The human cDNA clones contain a single open-reading frame encoding a protein of 144 amino acids with a predicted molecular mass of 16,293 daltons and show 69% nucleotide homology and 54% amino acid homology to mouse GM-CSF. The gene for human GM-CSF appears to exist as a single-copy gene.1 Human GM-CSF is a 22,000-dalton glycoprotein that stimulates the growth of myeloid progenitor cells and acts directly on mature neutrophils. The GM-CSF gene is localized by somatic cell hybrid analysis and in situ hybridization to human chromosome region 5q21-5q32, which is involved in interstitial deletions in the 5q- syndrome and acute myelogenous leukemia.2 A complementary DNA for the T lymphocyte-derived lymphokine, GM-CSF has been cloned, and recombinant GM-CSF protein has been expressed in yeast and purified to homogeneity. This purified human recombinant GM-CSF stimulates peripheral blood monocytes in vitro to become cytotoxic for the malignant melanoma cell line A375.3

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for GM-CSF

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Alternative Name: GM-CSF

Gene ID: 116630

UniProt: P48750

Pathways: JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin