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ICAM1 ELISA Kit

This Rat ICAM1 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Rat ICAM1.
Catalog No. ABIN2042493

Quick Overview for ICAM1 ELISA Kit (ABIN2042493)

Target

See all ICAM1 ELISA Kits
ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

Reactivity

  • 20
  • 12
  • 11
  • 4
  • 4
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 1
Rat

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

31.2-2000 pg/mL

Application

ELISA
  • Minimum Detection Limit

    31.2 pg/mL

    Cross-Reactivity (Details)

    There is no detectable cross-reactivity with other relevant proteins.

    Sensitivity

    < 2 pg/mL

    Components

    96-well plate pre-coated with anti-ICAM-1, Rat
    Lyophilized recombinant ICAM-1, Rat standard, 10 ng/tube, 2Ea
    Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
    Sample diluent buffer, 30 mL
    Antibody diluent buffer, 10 mL
    ABC diluent buffer, 10 mL
    TMB color developing agent, 10 mL
    TMB stop solution, 10 mL
  • Plate

    Pre-coated

    Protocol

    Sandwich ELISA quantitative detection kit for Rat ICAM-1 in cell culture supernates, serum and plasma (heparin, EDTA).Intercellular adhesion molecule-1 (ICAM-1) is an integral membrane protein, a member of the immunoglobulin superfamily, and a ligand for lymphocyte function-associated (LFA) antigens, a beta 2 leukocyte integrin.1 The normal function of human intercellular adhesion molecule-1 (ICAM-1) is to provide adhesion between endothelial cells and leukocytes after injury or stress. ICAM-1 binds to leukocyte function-associated antigen (LFA-1) or macrophage-1 antigen (Mac-1).2 ICAM-1 is found on leukocytes, fibroblasts, epithelial cells and endothelial cells and its expression is regulated by inflammatory cytokines.3 ICAM-1 has a tissue distribution similar to that of the major histocompatibility complex class II antigens and is likely to play a role in inflammatory responses.1 The standard product used in this kit is produced by recombinant gene expression, which is a dimeric protein linked with disulfide bonds.

    Restrictions

    For Research Use only
  • Storage

    4 °C/-20 °C
  • Target See all ICAM1 ELISA Kits

    ICAM1 (Intercellular Adhesion Molecule 1 (ICAM1))

    Alternative Name

    ICAM-1

    Target Type

    Viral Protein

    Gene ID

    3383

    UniProt

    P05362

    Pathways

    Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Regulation of Leukocyte Mediated Immunity, Thromboxane A2 Receptor Signaling
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