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IGFBPI ELISA Kit

This Colorimetric ELISA kit is designed for the quantitative measurement of Human IGFBPI.
Catalog No. ABIN2042504

Quick Overview for IGFBPI ELISA Kit (ABIN2042504)

Target

See all IGFBPI ELISA Kits
IGFBPI (Insulin-Like Growth Factor Binding Protein 1 (IGFBPI))

Reactivity

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Human

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

31.2-2000 pg/mL

Application

ELISA
  • Minimum Detection Limit

    31.2 pg/mL

    Cross-Reactivity (Details)

    There is no detectable cross-reactivity with other relevant proteins.

    Sensitivity

    < 1 pg/mL

    Components

    96-well plate pre-coated with anti-IGFBP-1, Human
    Lyophilized recombinant IGFBP-1, Human standard, 10 ng/tube, 2Ea
    Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
    Sample diluent buffer, 30 mL
    Antibody diluent buffer, 10 mL
    ABC diluent buffer, 10 mL
    TMB color developing agent, 10 mL
    TMB stop solution, 10 mL
  • Plate

    Pre-coated

    Protocol

    Sandwich ELISA quantitative detection kit for Human IGFBP-1 in cell culture supernates, serum, plasma (heparin, EDTA) and urine.IGFBP1 is also known as amniotic fluid binding protein (AFBP), placental protein-12, alpha-pregnancy-associated endometrial globulin, growth hormone independent binding protein, binding protein-28, binding protein-26, and binding protein-25.1 The low-molecular weight insulin-like growth-factor binding protein (IGF-BP25) is synthesized by human liver, secretory endometrium and decidua, and is also present in human serum. It binds insulin-like growth factors IGF-I and IGF-II with high affinity, and is proposed to act as a paracrine regulator of cell growth.2 These IGF-binding proteins are expressed at different concentrations in different tissues and are thought to regulate the activity of IGF I and II.3 The gene is organized in four exons and spans 5.9 kb.4 The IBP-1 gene is a single copy gene, located on chromosome 7.5 The standard product used in this kit is recombinant human IGFBP-1, consisting of 234 amino acids with the molecular mass of 25kD.

    Restrictions

    For Research Use only
  • Storage

    4 °C/-20 °C
  • Target See all IGFBPI ELISA Kits

    IGFBPI (Insulin-Like Growth Factor Binding Protein 1 (IGFBPI))

    Alternative Name

    IGFBP-1

    Gene ID

    3484

    UniProt

    P08833

    Pathways

    Myometrial Relaxation and Contraction, ER-Nucleus Signaling, Growth Factor Binding
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