IGFBPI ELISA Kit
Quick Overview for IGFBPI ELISA Kit (ABIN2042505)
Target
See all IGFBPI ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
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Minimum Detection Limit
- 31.2 pg/mL
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Cross-Reactivity (Details)
- There is no detectable cross-reactivity with other relevant proteins.
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Sensitivity
- < 3 pg/mL
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Components
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96-well plate pre-coated with anti-IGFBP-1, Mouse
Lyophilized recombinant IGFBP-1, Mouse standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
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Plate
- Pre-coated
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Protocol
- Sandwich ELISA quantitative detection kit for Mouse IGFBP-1 in cell culture supernates and serum.IGFBP1 is also known as amniotic fluid binding protein (AFBP), placental protein-12, alpha-pregnancy-associated endometrial globulin, growth hormone independent binding protein, binding protein-28, binding protein-26, and binding protein-25.1 The low-molecular weight insulin-like growth-factor binding protein (IGF-BP25) is synthesized by human liver, secretory endometrium and decidua, and is also present in human serum. It binds insulin-like growth factors IGF-I and IGF-II with high affinity, and is proposed to act as a paracrine regulator of cell growth.2 These IGF-binding proteins are expressed at different concentrations in different tissues and are thought to regulate the activity of IGF I and II.3 The gene is organized in four exons and spans 5.9 kb.4 The IBP-1 gene is a single copy gene, located on chromosome 7.5 The standard product used in this kit is recombinant mouse IGFBP-1, consisting of 247 amino acids with the molecular mass of 27kD.
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Restrictions
- For Research Use only
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Storage
- 4 °C/-20 °C
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- IGFBPI (Insulin-Like Growth Factor Binding Protein 1 (IGFBPI))
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Alternative Name
- IGFBP-1
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Gene ID
- 16006
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UniProt
- P47876
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Pathways
- Myometrial Relaxation and Contraction, ER-Nucleus Signaling, Growth Factor Binding
Target See all IGFBPI ELISA Kits
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