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IGFBPI ELISA Kit

This Mouse IGFBPI ELISA Kit is a Colorimetric ELISA Kit designed to quantify Mouse IGFBPI.
Catalog No. ABIN2042505

Quick Overview for IGFBPI ELISA Kit (ABIN2042505)

Target

See all IGFBPI ELISA Kits
IGFBPI (Insulin-Like Growth Factor Binding Protein 1 (IGFBPI))

Reactivity

  • 11
  • 7
  • 4
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Mouse

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

31.2-2000 pg/mL

Application

ELISA
  • Minimum Detection Limit

    31.2 pg/mL

    Cross-Reactivity (Details)

    There is no detectable cross-reactivity with other relevant proteins.

    Sensitivity

    < 3 pg/mL

    Components

    96-well plate pre-coated with anti-IGFBP-1, Mouse
    Lyophilized recombinant IGFBP-1, Mouse standard, 10 ng/tube, 2Ea
    Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
    Sample diluent buffer, 30 mL
    Antibody diluent buffer, 10 mL
    ABC diluent buffer, 10 mL
    TMB color developing agent, 10 mL
    TMB stop solution, 10 mL
  • Plate

    Pre-coated

    Protocol

    Sandwich ELISA quantitative detection kit for Mouse IGFBP-1 in cell culture supernates and serum.IGFBP1 is also known as amniotic fluid binding protein (AFBP), placental protein-12, alpha-pregnancy-associated endometrial globulin, growth hormone independent binding protein, binding protein-28, binding protein-26, and binding protein-25.1 The low-molecular weight insulin-like growth-factor binding protein (IGF-BP25) is synthesized by human liver, secretory endometrium and decidua, and is also present in human serum. It binds insulin-like growth factors IGF-I and IGF-II with high affinity, and is proposed to act as a paracrine regulator of cell growth.2 These IGF-binding proteins are expressed at different concentrations in different tissues and are thought to regulate the activity of IGF I and II.3 The gene is organized in four exons and spans 5.9 kb.4 The IBP-1 gene is a single copy gene, located on chromosome 7.5 The standard product used in this kit is recombinant mouse IGFBP-1, consisting of 247 amino acids with the molecular mass of 27kD.

    Restrictions

    For Research Use only
  • Storage

    4 °C/-20 °C
  • Target See all IGFBPI ELISA Kits

    IGFBPI (Insulin-Like Growth Factor Binding Protein 1 (IGFBPI))

    Alternative Name

    IGFBP-1

    Gene ID

    16006

    UniProt

    P47876

    Pathways

    Myometrial Relaxation and Contraction, ER-Nucleus Signaling, Growth Factor Binding
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