IL12B ELISA Kit

Catalog No. ABIN2042520

This Mouse IL12B ELISA Kit is a Colorimetric ELISA Kit designed to quantify Mouse IL12B.

Quick Overview for IL12B ELISA Kit (ABIN2042520)

Target: IL12B (Interleukin 12 beta (IL12B))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6-1000 pg/mL

Application: ELISA

Product Details IL12B ELISA Kit

Minimum Detection Limit: 15.6 pg/mL

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components: 96-well plate pre-coated with anti-IL-12(p40), Mouse
Lyophilized recombinant IL-12(p40), Mouse standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Application Details

Plate: Pre-coated

Protocol: Sandwich ELISA quantitative detection kit for Mouse IL-12(p40) in cell culture supernates, serum and plasma (heparin, EDTA, citrate).Interleukin-12 (IL12, formerly NKSF, for natural killer cell stimulatory factor, or CLMF, for cytotoxic lymphocyte maturation factor) is a novel cytokine cloned from B-cell lines. IL-12 is a heterodimeric molecule composed of p35 and p40 subunits.1 The larger 40kD subunit (p40) is a member of the cytokine receptor family, and the smaller 35kD subunit (p35) is related to IL6 and GCSF.2 Both IL-12 p40(-/-) and p35(-/-) mice fail to produce IL-12 p70 heterodimer.3 Interleukin (IL)-12 has been cloned on the basis of its ability to activate natural killer (NK) cells and promote the development of cytolytic T cells. With further understanding of its activities, IL-12 has emerged as an important cytokine, affecting both immune and hematologic functions. It has been shown to be necessary for the T cell independent induction of interferon (IFN)-gamma, critical for the initial suppression of bacterial and parasitic infection, for the development of a Th1 response, critical for effective host defense against intracellular pathogens, and for the activation of differentiated T lymphocytes of both CD4+ and CD8+ phenotype.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for IL12B

Target: IL12B (Interleukin 12 beta (IL12B))

Alternative Name: IL-12(p40)

Gene ID: 16160

UniProt: P43432

Pathways: JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation