IL-15 ELISA Kit

Catalog No. ABIN2042525

This Colorimetric ELISA kit is designed for the quantitative measurement of Human IL-15.

Quick Overview for IL-15 ELISA Kit (ABIN2042525)

Target: IL-15 (IL15) (Interleukin 15 (IL15))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6-1000 pg/mL

Application: ELISA

Product Details IL-15 ELISA Kit

Minimum Detection Limit: 15.6 pg/mL

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 3 pg/mL

Components: 96-well plate pre-coated with anti-IL-15, Human
Lyophilized recombinant IL-15, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Application Details

Plate: Pre-coated

Protocol: Sandwich ELISA quantitative detection kit for Human IL-15 in cell culture supernates, serum and plasma (heparin, EDTA).Interleukin (IL)-15 is a cytokine with the ability to stimulate the proliferation activity of Th1 and/or Th2 lymphocytes. IL-15 is a novel cytokine whose effects on T-cell activation and proliferation are similar to those of interleukin-2 (IL-2), presumably because IL-15 utilizes the beta and gamma chains of the IL-2 receptor.1 IL-15 can play a role in the initiation and outcome of acute and chronic rejection. Anti-IL-15 therapy in combination with classic immunosuppression therapy might thus be beneficial in the prevention of acute, and especially chronic, allograft rejection.2 The human IL15 gene is mapped to human chromosome 4q31 by fluorescence in situ hybridization.1 The standard product used in this kit is recombinant human IL-15, consisting of 114 amino acids with the molecular mass of 12.9kD.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for IL-15

Target: IL-15 (IL15) (Interleukin 15 (IL15))

Alternative Name: IL-15

Gene ID: 3600

UniProt: P40933

Pathways: JAK-STAT Signaling, Glycosaminoglycan Metabolic Process