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IL-2 ELISA Kit

This Mouse IL-2 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Mouse IL-2.
Catalog No. ABIN2042535

Quick Overview for IL-2 ELISA Kit (ABIN2042535)

Target

See all IL-2 (IL2) ELISA Kits
IL-2 (IL2) (Interleukin 2 (IL2))

Reactivity

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  • 2
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Mouse

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

15.6-1000 pg/mL

Application

ELISA
  • Minimum Detection Limit

    15.6 pg/mL

    Cross-Reactivity (Details)

    There is no detectable cross-reactivity with other relevant proteins.

    Sensitivity

    < 1 pg/mL

    Components

    96-well plate pre-coated with anti-IL-2, Mouse
    Lyophilized recombinant IL-2, Mouse standard, 10 ng/tube, 2Ea
    Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
    Sample diluent buffer, 30 mL
    Antibody diluent buffer, 10 mL
    ABC diluent buffer, 10 mL
    TMB color developing agent, 10 mL
    TMB stop solution, 10 mL
  • Plate

    Pre-coated

    Protocol

    Sandwich ELISA quantitative detection kit for Mouse IL-2 in cell culture supernates, serum and plasma (heparin, EDTA, citrate.Interleukin-2 (IL2), formerly referred to as T-cell growth factor, is a powerfully immunoregulatory lymphokine that is produced by lectin- or antigen-activated T cells. It is produced not only by mature T lymphocytes on stimulation but also constitutively by certain T-cell lymphoma cell lines. The lymphokine interleukin-2 (IL-2) is responsible for autocrine cell cycle progression and regulation of immune responses. IL-2 expression in mature thymocytes and T cells has been found to be tightly controlled by monoallelic expression.1 IL-2 can act as a growth hormone for both B and T lymphocytes.2 The human gene for interleukin 2 (IL2) is assigned to chromosome 4.3 Mouse IL-2 contains 149 amino acids in mature form with the molecular mass of 17.2kD.

    Restrictions

    For Research Use only
  • Storage

    4 °C/-20 °C
  • Target See all IL-2 (IL2) ELISA Kits

    IL-2 (IL2) (Interleukin 2 (IL2))

    Alternative Name

    IL-2

    Gene ID

    16183

    UniProt

    P04351

    Pathways

    JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation
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