IL-3 ELISA Kit
Quick Overview for IL-3 ELISA Kit (ABIN2042547)
Target
See all IL-3 ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
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Minimum Detection Limit
- 7.8 pg/mL
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Cross-Reactivity (Details)
- There is no detectable cross-reactivity with other relevant proteins.
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Sensitivity
- < 1 pg/mL
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Components
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96-well plate pre-coated with anti-IL-3, Mouse
Lyophilized recombinant IL-3, Mouse standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
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Interleukin 3 (IL-3) ELISA Kit
IL-3 Reactivity: Mouse Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 3 (IL-3) ELISA KitIL-3 Reactivity: Chicken Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 3 (IL-3) ELISA KitIL-3 Reactivity: Cow Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 3 (IL-3) ELISA KitIL-3 Reactivity: Human Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 3 (IL-3) ELISA KitIL-3 Reactivity: Rat Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 3 (IL-3) ELISA KitIL-3 Reactivity: Dog Colorimetric Sandwich ELISA 15.62 pg/mL - 1000 pg/mL Plasma, Serum
Interleukin 3 (IL-3) ELISA KitSmall Sample IL-3 Reactivity: Human Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 3 (IL-3) ELISA KitIL-3 Reactivity: Mouse AA 27-166 Colorimetric Sandwich ELISA 7.8-500 pg/mL Cell Culture Supernatant, Serum
Interleukin 3 (IL-3) ELISA KitIL-3 Reactivity: Human AA 20-152 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Interleukin 3 (IL-3) ELISA KitIL-3 Reactivity: Rat AA 27-169 Colorimetric Sandwich ELISA 7.8-500 pg/mL Cell Culture Supernatant, Serum
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Plate
- Pre-coated
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Protocol
- Sandwich ELISA quantitative detection kit for Mouse IL-3 in cell culture supernates and serum.The gene IL-3 encodes interleukin 3, a hematopoietic colony-stimulating factor (CSF) that is capable of supporting the proliferation of a broad range of hematopoietic cell types.1 Interleukin-3 (IL-3), a protein of 140 amino acids, is chemically synthesized by means of an automated peptide synthesizer and is shown to have the biological activities attributed to native IL-3.2 The cDNA sequence for murine interleukin-3, one of the colony stimulating factors that regulate haematopoiesis, codes for a polypeptide of 166 amino acids including a putative signal peptide.3 The mouse IL 3 gene is located on chromosome 11.4 The human gene encoding IL 3 is tandemly arrayed on the long arm of chromosome 5.5 The standard product used in this kit is recombinant mouse IL-3, consisting of 135 amino acids with the molecular mass of 15kD.
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Restrictions
- For Research Use only
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Storage
- 4 °C/-20 °C
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- IL-3 (Interleukin 3 (IL-3))
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Alternative Name
- IL-3
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Gene ID
- 16187
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UniProt
- P01586
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Pathways
- JAK-STAT Signaling, Regulation of Carbohydrate Metabolic Process, Autophagy
Target See all IL-3 ELISA Kits
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