IL-5 ELISA Kit

Catalog No. ABIN2042556

This Mouse IL-5 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Mouse IL-5.

Quick Overview for IL-5 ELISA Kit (ABIN2042556)

Target: IL-5 (IL5) (Interleukin 5 (IL5))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6-1000 pg/mL

Application: ELISA

Product Details IL-5 ELISA Kit

Minimum Detection Limit: 15.6 pg/mL

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 2 pg/mL

Components: 96-well plate pre-coated with anti-IL-5, Mouse
Lyophilized recombinant IL-5, Mouse standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Application Details

Plate: Pre-coated

Protocol: Sandwich ELISA quantitative detection kit for Mouse IL-5 in cell culture supernates and serum.Interleukin-5 (IL-5) is also known as eosinophil differentiation factor (EDF). IL-5 is a potential candidate gene in the pathogenesis of asthma, as it is the main cytokine controlling eosinophil activity and eosinophils are pivotal in the development of airway inflammation.1 The predicted amino acid sequence of 134 amino acids is identical with that recently reported for human interleukin-5 but shows no significant homology with other known hemopoietic growth regulators.2 IL-5 is a lineage-specific hematopoietic growth factor that stimulates the production of eosinophils and eosinophil colonies from normal human bone marrow cells. IL-5 gene to human chromosome 5 at bands q23-31.3 Human and mouse of IL-5 share 70% amino acid sequence homology.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for IL-5

Target: IL-5 (IL5) (Interleukin 5 (IL5))

Alternative Name: Interleukin 5

Gene ID: 16191

UniProt: P04401

Pathways: JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Production of Molecular Mediator of Immune Response, Feeding Behaviour