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IL-7 ELISA Kit

This Colorimetric ELISA kit is designed for the quantitative measurement of Human IL-7.
Catalog No. ABIN2042561

Quick Overview for IL-7 ELISA Kit (ABIN2042561)

Target

See all IL-7 (IL7) ELISA Kits
IL-7 (IL7) (Interleukin 7 (IL7))

Reactivity

  • 10
  • 8
  • 7
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Human

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

15.6-1000 pg/mL

Application

ELISA
  • Minimum Detection Limit

    15.6 pg/mL

    Cross-Reactivity (Details)

    There is no detectable cross-reactivity with other relevant proteins.

    Sensitivity

    < 1 pg/mL

    Components

    96-well plate pre-coated with anti-IL-7, Human
    Lyophilized recombinant IL-7, Human standard, 10 ng/tube, 2Ea
    Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
    Sample diluent buffer, 30 mL
    Antibody diluent buffer, 10 mL
    ABC diluent buffer, 10 mL
    TMB color developing agent, 10 mL
    TMB stop solution, 10 mL
  • Plate

    Pre-coated

    Protocol

    Sandwich ELISA quantitative detection kit for Human Interleukin 7 in cell culture supernates, serum and plasma (EDTA).IL-7 a hematopoietic growth factor secreted by stromal cells in the red marrow and thymus. It is also produced by keratinocytes,1 dendritic cells,2 hepatocytes,3 neurons, and endothelial cells4 . Interleukin 7 (IL7) is a protein5 that in humans is encoded by the IL7 gene.6 7 8Knockout studies in mice suggested that this cytokine plays an essential role in lymphoid cell survival.9 IL-7 is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. This cytokine is found to be a cofactor for V(D)J rearrangement of the T cell receptor beta (TCRB) during early T cell development.10

    Restrictions

    For Research Use only
  • Storage

    4 °C/-20 °C
  • Target See all IL-7 (IL7) ELISA Kits

    IL-7 (IL7) (Interleukin 7 (IL7))

    Alternative Name

    IL-7

    Gene ID

    3574

    UniProt

    A8K673

    Pathways

    JAK-STAT Signaling
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