Leptin ELISA Kit

Catalog No. ABIN2042576

This Colorimetric ELISA kit is designed for the quantitative measurement of Human Leptin.

Quick Overview for Leptin ELISA Kit (ABIN2042576)

Target: Leptin (LEP)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 62.5-4000 pg/mL

Application: ELISA

Product Details Leptin ELISA Kit

Minimum Detection Limit: 62.5 pg/mL

Brand: BioAssay™

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components: A: Microtiter Plate, 1x96 wells (12 x 8 strips). Pre-coated with anti-human Leptin antibody.
B: Leptin Standard, 2x10 ng. Lyophilized recombinant human Leptin.
C: Biotinylated anti-Leptin Antibody, 1x130 µL
D: ABC Complex (Avidin-Biotin-Peroxidase Complex), 1x130 µL
E: Sample Diluent Buffer, 1x30 mL
F: Antibody Diluent Buffer, 1x10 mL
G: ABC Diluent Buffer, 1x10 mL
H: TMB Substrate, 1x10 mL
I: TMB Stop Solution, 1x10 mL

Application Details

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for Leptin

Target: Leptin (LEP)

Alternative Name: Leptin

Background: Leptin (or obese, OB) is a circulating hormone that is expressed abundantly and specifically in the adipose tissue.1 Human ob protein is a 166-amino acid polypeptide with a putative signal sequence and is 84 and 83 % homologous to the mouse and rat ob proteins, respectively.2 Northern blot analysis reveals that OB RNA is present at a high level in adipose tissue but at much lower levels in placenta and heart.3 Indeed, Leptin is first described as an adipocyte-derived signalling factor, which, after interaction with its receptors, induces a complex response including control of bodyweight and energy expenditure. Leptin seems in addition to its role in metabolic control to have important roles in reproduction and neuroendocrine signalling.4 Leptin is a powerful inhibitor of bone formation in vivo.5 Comparative mapping of mouse and human DNA indicates that the ob gene is located within a region of mouse chromosome 6 that is homologous to a portion of human chromosome 7q.3 The standard product used in this kit is recombinant human Leptin.

Gene ID: 3952

UniProt: A4D0Y8

Pathways: JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process