Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

M-CSF/CSF1 ELISA Kit

Mouse M-CSF/CSF1 ELISA Kit Colorimetric assay for quantification of Mouse M-CSF/CSF1.
Catalog No. ABIN2042584

Quick Overview for M-CSF/CSF1 ELISA Kit (ABIN2042584)

Target

See all M-CSF/CSF1 (CSF1) ELISA Kits
M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))

Reactivity

  • 12
  • 8
  • 4
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Mouse

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

15.6-1000 pg/mL

Application

ELISA
  • Minimum Detection Limit

    15.6 pg/mL

    Cross-Reactivity (Details)

    There is no detectable cross-reactivity with other relevant proteins.

    Sensitivity

    < 1 pg/mL

    Components

    96-well plate pre-coated with anti-M-CSF, Mouse
    Lyophilized recombinant M-CSF, Mouse standard, 10 ng/tube, 2Ea
    Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
    Sample diluent buffer, 30 mL
    Antibody diluent buffer, 10 mL
    ABC diluent buffer, 10 mL
    TMB color developing agent, 10 mL
    TMB stop solution, 10 mL
  • Plate

    Pre-coated

    Protocol

    Sandwich ELISA quantitative detection kit for Mouse M-CSF in cell culture supernates, tissue homogenates, serum and plasma (EDTA).M-CSF, also called CSF1, consists of a 14-amino acid peptide, longer than the usual 8-to-11 mer recognized by most CTLs. The M-CSF gene is mapped to 1p21-p13 and contains 10 exons and 9 introns spanning 20 kb1. Although it is a single-copy gene, its expression results in the synthesis of several mRNAs, ranging in size from about 1.5 to 4.5 kb2. There are 2 forms of M-CSF, with 224 and 522 amino acids, resulting from alternative splicing3. Furthermore, M-CSF has a role in development of the placenta. Uterine M-CSF concentration is regulated by a synergistic action of estradiol and progesterone. M-CSF is produced by uterine glandular epithelial cells. It had been found that FMS, the M-CSF receptor, is expressed in placenta and choriocarcinoma cell lines4.

    Restrictions

    For Research Use only
  • Storage

    4 °C/-20 °C
  • Target See all M-CSF/CSF1 (CSF1) ELISA Kits

    M-CSF/CSF1 (CSF1) (Colony Stimulating Factor 1 (Macrophage) (CSF1))

    Alternative Name

    M-CSF

    Gene ID

    12977

    UniProt

    P07141

    Pathways

    RTK Signaling
You are here:
Chat with us!