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CCL2 ELISA Kit

Mouse CCL2 ELISA Kit Colorimetric assay for quantification of Mouse CCL2.
Catalog No. ABIN2042587

Quick Overview for CCL2 ELISA Kit (ABIN2042587)

Target

See all CCL2 ELISA Kits
CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Reactivity

  • 18
  • 17
  • 17
  • 5
  • 5
  • 5
  • 4
  • 4
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
Mouse

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

15.6-1000 pg/mL

Application

ELISA
  • Minimum Detection Limit

    15.6 pg/mL

    Cross-Reactivity (Details)

    There is no detectable cross-reactivity with other relevant proteins.

    Sensitivity

    < 6 pg/mL

    Components

    96-well plate pre-coated with anti-MCP-1, Mouse
    Lyophilized recombinant MCP-1, Mouse standard, 10 ng/tube, 2Ea
    Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
    Sample diluent buffer, 30 mL
    Antibody diluent buffer, 10 mL
    ABC diluent buffer, 10 mL
    TMB color developing agent, 10 mL
    TMB stop solution, 10 mL
  • Plate

    Pre-coated

    Protocol

    Sandwich ELISA quantitative detection kit for Mouse MCP-1 in cell culture supernates and serum.Monocyte chemotactic protein-1 (MCP-1) is a member of the small inducible gene (SIG) family. It has been shown to play a role in the recruitment of monocytes to sites of injury and infection. By analysis of a panel of somatic cell hybrids, we have localized the MCP-1 gene, designated SCYA2, to human chromosome 17. In situ hybridization confirmed this assignment and further localized the gene to 17q11.2-q21.1.1 MCP-1 plays a unique and crucial role in the initiation of atherosclerosis and may provide a new therapeutic target in this disorder.2 Monocyte chemoattractant protein-1 (MCP-1), regulated on activation, normal T cell expressed and secreted,and stimulation of monocytes from healthy carriers of the genotype GG with Mycobacterium tuberculosis antigens yielded higher MCP-1.3 The standard used in this kit is recombinant mouse MCP-1(Q24-R96) with the molecular mass of 8.5kD.

    Restrictions

    For Research Use only
  • Storage

    4 °C/-20 °C
  • Target See all CCL2 ELISA Kits

    CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

    Alternative Name

    MCP-1

    Gene ID

    20296

    UniProt

    P10148

    Pathways

    Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, ER-Nucleus Signaling, Unfolded Protein Response, The Global Phosphorylation Landscape of SARS-CoV-2 Infection
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