CCL2 ELISA Kit

Catalog No. ABIN411320

This Colorimetric ELISA kit is designed for the quantitative measurement of Human CCL2. There are 33 publications available.

Quick Overview for CCL2 ELISA Kit (ABIN411320)

Target: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Binding Specificity: AA 24-99

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6 pg/mL - 1000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate), Urine

Product Details CCL2 ELISA Kit

Minimum Detection Limit: 15.6 pg/mL

Purpose: Sandwich Human MCP-1 / CCL2 ELISA Kit to quantitate Human CCL2 in cell culture supernatants, serum, plasma (heparin, EDTA, citrate) and urine.

Analytical Method: Quantitative

Specificity: Expression system for standard: E.coli
Immunogen sequence: Q24-T99

Capture antibody and Detection antibody: polyclonal antibody from goat, polyclonal antibody from goat

Cross-Reactivity (Details): There is cross-reactivity with human Eotaxin, MCP-3<1 % .

Sensitivity: < 1 pg/mL

Components:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Material not included: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Sample Volume: 100 μL

Plate: Pre-coated

Protocol: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Assay Precision: Intra-Assay Precision (CV%): 5%, 5.5%, 6.9%
Inter-Assay Precision (CV%): 6.6%, 6.6%, 7.8%

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Expiry Date: 12 months

References for CCL2 ELISA Kit (ABIN411320)

Namlı Kalem, Akgun, Kalem, Bakirarar, Celik: "Chemokine (C-C motif) ligand-2 (CCL2) and oxidative stress markers in recurrent pregnancy loss and repeated implantation failure." in: Journal of assisted reproduction and genetics, Vol. 34, Issue 11, pp. 1501-1506, (2018) (PubMed).

Sun, Chen, Wang, Wan, Zhang, Zhang, Lin, Zhang: "Salusin-β Is Involved in Diabetes Mellitus-Induced Endothelial Dysfunction via Degradation of Peroxisome Proliferator-Activated Receptor Gamma." in: Oxidative medicine and cellular longevity, Vol. 2017, pp. 6905217, (2018) (PubMed).

Capistrano, Meneses, de Oliveira Neves, de Almeida Leitão, Martins, Libório: "Renal Evaluation in Common Variable Immunodeficiency." in: Journal of immunology research, Vol. 2018, pp. 5841031, (2018) (PubMed).

Blanchard, Stepanovska, Starck, Erhardt, Römer, Meyer Zu Heringdorf, Pfeilschifter, Zangemeister-Wittke, Huwiler: "Downregulation of the S1P Transporter Spinster Homology Protein 2 (Spns2) Exerts an Anti-Fibrotic and Anti-Inflammatory Effect in Human Renal Proximal Tubular Epithelial Cells." in: International journal of molecular sciences, Vol. 19, Issue 5, (2018) (PubMed).

Liao, Cai, Xu, Wang, Qiu, Xie, Huang, Sui: "Protective Role of Antioxidant Huskless Barley Extracts on TNF-α-Induced Endothelial Dysfunction in Human Vascular Endothelial Cells." in: Oxidative medicine and cellular longevity, Vol. 2018, pp. 3846029, (2018) (PubMed).

Bao, Li, Luo, Tang, Lv, Li, Zhou: "NF-κB-Regulated miR-99a Modulates Endothelial Cell Inflammation." in: Mediators of inflammation, Vol. 2016, pp. 5308170, (2017) (PubMed).

Sun, Zhu, Cai, Qiu: "Hypaphorine Attenuates Lipopolysaccharide-Induced Endothelial Inflammation via Regulation of TLR4 and PPAR-γ Dependent on PI3K/Akt/mTOR Signal Pathway." in: International journal of molecular sciences, Vol. 18, Issue 4, (2017) (PubMed).

Chen, Liu, Chen, Tseng, Shen, Hwang, Hsu: "Oligo-Fucoidan prevents IL-6 and CCL2 production and cooperates with p53 to suppress ATM signaling and tumor progression." in: Scientific reports, Vol. 7, Issue 1, pp. 11864, (2017) (PubMed).

Liu, Li, Liu, Wang, Jia: "Inhibition of zymosan-induced cytokine and chemokine expression in human corneal fibroblasts by triptolide." in: International journal of ophthalmology, Vol. 9, Issue 1, pp. 9-14, (2016) (PubMed).

Aizman, Vinodkumar, McGrogan, Bates: "Cell Injury-Induced Release of Fibroblast Growth Factor 2: Relevance to Intracerebral Mesenchymal Stromal Cell Transplantations." in: Stem cells and development, Vol. 24, Issue 14, pp. 1623-34, (2015) (PubMed).

Jiang, Zhang, Xiong, Jia, Sun, Lin, Shen, Xie, Yan: "EETs Attenuate Ox-LDL-Induced LTB4 Production and Activity by Inhibiting p38 MAPK Phosphorylation and 5-LO/BLT1 Receptor Expression in Rat Pulmonary Arterial Endothelial Cells." in: PLoS ONE, Vol. 10, Issue 6, pp. e0128278, (2015) (PubMed).

Tu, Chen, Yang, Qi, Lu, Zhao: "Amyloid-? Activates Microglia and Regulates Protein Expression in a Manner Similar to Prions." in: Journal of molecular neuroscience : MN, Vol. 56, Issue 2, pp. 509-18, (2015) (PubMed).

Zhang, Jiang, Chen, Wang, Feng, Tao, Qin: "Serum Levels of IL-1 ? , IL-6, TGF- ? , and MMP-9 in Patients Undergoing Carotid Artery Stenting and Regulation of MMP-9 in a New In Vitro Model of THP-1 Cells Activated by Stenting." in: Mediators of inflammation, Vol. 2015, pp. 956082, (2015) (PubMed).

Han, Wang, Zhang, Liu, Li, Wang, Song, Pang, Ouyang, Tang: "Apolipoprotein CIII regulates lipoprotein-associated phospholipase A2 expression via the MAPK and NF?B pathways." in: Biology open, Vol. 4, Issue 5, pp. 661-5, (2015) (PubMed).

Dai, Wu, He, Zhang, Chen, Xu, Wang, Gruzdev, Zeldin, Wang: "Epoxyeicosatrienoic acids regulate macrophage polarization and prevent LPS-induced cardiac dysfunction." in: Journal of cellular physiology, Vol. 230, Issue 9, pp. 2108-19, (2015) (PubMed).

Li, Wang, Zhang, Ma, Zhao: "IL-1β mediates MCP-1 induction by Wnt5a in gastric cancer cells." in: BMC cancer, Vol. 14, pp. 480, (2015) (PubMed).

Liu, Wang, Ding, Wang, Zeng: "A potential therapeutic effect of CYP2C8 overexpression on anti-TNF-α activity." in: International journal of molecular medicine, Vol. 34, Issue 3, pp. 725-32, (2015) (PubMed).

Meneses, Libório, de Daher, da Silva, da Costa, Pontes, Martins: "Urinary monocyte chemotactic protein-1 (MCP-1) in leprosy patients: increased risk for kidney damage." in: BMC infectious diseases, Vol. 14, pp. 451, (2014) (PubMed).

Xu, Kong, Zhou, Zhang, Liu, Yan, Xie, Xie: "oxLDL/?2GPI/anti-?2GPI complex induced macrophage differentiation to foam cell involving TLR4/NF-kappa B signal transduction pathway." in: Thrombosis research, Vol. 134, Issue 2, pp. 384-92, (2014) (PubMed).

Tu, Yang, Zhou, Qi, Wang, Kouadir, Xu, Yin, Zhao: "PrP106-126 and A? 1-42 peptides induce BV-2 microglia chemotaxis and proliferation." in: Journal of molecular neuroscience : MN, Vol. 52, Issue 1, pp. 107-16, (2014) (PubMed).

Target Details for CCL2

Target: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Alternative Name: CCL2

Background:

Background: Monocyte chemoattractant protein-1 (MCP-1), a member of the chemokine (chemotactic cytokine) family, is a potent monocyte agonist that is upregulated by oxidized lipids. MCP-1 is also known as CCL2, SCYA2, MCAF. MCAF is a member of family of factors involved in immune and inflammatory responses. The amino acid sequence deduced from the nucleotide sequence reveals the primary structure of the MCAF precursor to be composed of a putative signal peptide sequence of 23 amino acid residues and a mature MCAF sequence of 76 amino acid residues. MCP-1 plays a unique and crucial role in the initiation of atherosclerosis and may provide a new therapeutic target in this disorder. Human MCP-1 is a 8.7KDa non-glycoprotein, consisting of 99 amino acids in precursor form and 76 amino acids in mature form.

Gene Full Name: C-C motif chemokine ligand 2

Gene ID: 6347

UniProt: P13500

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, ER-Nucleus Signaling, Unfolded Protein Response, The Global Phosphorylation Landscape of SARS-CoV-2 Infection