MICB ELISA Kit

Catalog No. ABIN2042594

This Colorimetric ELISA kit is designed for the quantitative measurement of Human MICB.

Quick Overview for MICB ELISA Kit (ABIN2042594)

Target: MICB (MHC Class I Polypeptide-Related Sequence B (MICB))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5-4000 pg/mL

Application: ELISA

Product Details MICB ELISA Kit

Minimum Detection Limit: 62.5 pg/mL

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 10 pg/mL

Components: 96-well plate pre-coated with anti-MICB, Human
Lyophilized recombinant MICB, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Application Details

Plate: Pre-coated

Protocol: Sandwich ELISA quantitative detection kit for Human MICB in cell culture supernates, serum and plasma (heparin, EDTA).MHC class I polypeptide-related sequence B, also called MICB is a protein that in humans is encoded by the MICB gene. The MICB gene is mapped to 6p21.33. This gene encodes a heavily glycosylated protein which is a ligand for the NKG2D type II receptor. Binding of the ligand activates the cytolytic response of natural killer (NK) cells, CD8 alphabeta T cells, and gammadelta T cells which express the receptor. This protein is stress-induced and is similar to MHC class I molecules, however, it does not associate with beta-2-microglobulin or bind peptides. It seems to have no role in antigen presentation. This gene acts as a stress-induced self-antigen that is recognized by gamma delta T cells. It is the ligand for the KLRK1/NKG2D receptor and binds to KLRK1 leads to cell lysis.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for MICB

Target: MICB (MHC Class I Polypeptide-Related Sequence B (MICB))

Alternative Name: MICB

Gene ID: 4277

UniProt: Q29980

Pathways: Human Leukocyte Antigen (HLA) in Adaptive Immune Response