MMP2 ELISA Kit
Quick Overview for MMP2 ELISA Kit (ABIN2042604)
Target
See all MMP2 ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
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Minimum Detection Limit
- 6 pg/mL
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Cross-Reactivity (Details)
- There is no detectable cross-reactivity with other relevant proteins.
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Sensitivity
- < 10 pg/mL
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Components
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96-well plate pre-coated with anti-MMP-2, Rat
Lyophilized recombinant MMP-2, Rat standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
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Matrix Metalloproteinase 2 (MMP2) ELISA Kit
MMP2 Reactivity: Human Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 Reactivity: Rat Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 Reactivity: Mouse Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 Reactivity: Pig Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 Reactivity: Horse Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 Reactivity: Rat AA 30-662 Colorimetric Sandwich ELISA 625-40000 pg/mL Cell Culture Supernatant, Plasma (heparin), Serum
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 Reactivity: Human AA 30-660 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (heparin), Serum
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 Reactivity: Mouse AA 30-662 Colorimetric Sandwich ELISA 625-40000 pg/mL Cell Culture Supernatant, Plasma (heparin), Serum
SensoLyte® 520 MMP-2 Assay KitFRET Fluorometric
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 Reactivity: Human Colorimetric Sandwich ELISA 0.5 ng/mL - 32 ng/mL Plasma, Serum, Tissue Homogenate
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Plate
- Pre-coated
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Protocol
- Sandwich ELISA quantitative detection kit for Rat MMP-2 in cell culture supernates, serum, plasma (heparin).Type IV collagenase, 72kD, is officially designated matrix metalloproteinase-2 (MMP2). It is also known as gelatinase, 72kD. MMP-2 plays an essential role in angiogenesis and arteriogenesis, two processes critical to restoration of tissue perfusion after ischemia. MMP-2 expression is increased in tissue ischemia, but the responsible mechanisms remain unknown.1 Matrix metalloproteinases (MMPs) catalyze extracellular matrix degradation. Control of their activity is a promising target for therapy of diseases characterized by abnormal connective tissue turnover. MMPs are expressed as latent proenzymes that are activated by proteolytic cleavage that triggers a conformational change in the propeptide (cysteine switch). The structure of proMMP-2 reveals how the propeptide shields the catalytic cleft and that the cysteine switch may operate through cleavage of loops essential for propeptide stability.2 The gene is localized to 16q21 using somatic cell hybrids and in situ hybridization.3 The standard product used in this kit is recombinant rat MMP-2, consisting of 662 amino acids with the molecular mass of 72kD. The detected MMP-2 includes zymogen and active enzyme.
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Restrictions
- For Research Use only
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Storage
- 4 °C/-20 °C
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- MMP2 (Matrix Metalloproteinase 2 (MMP2))
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Alternative Name
- MMP-2
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Gene ID
- 81686
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UniProt
- P33436
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Pathways
- Activation of Innate immune Response
Target See all MMP2 ELISA Kits
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