MMP3 ELISA Kit
Quick Overview for MMP3 ELISA Kit (ABIN2042606)
Target
See all MMP3 ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
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Minimum Detection Limit
- 6 pg/mL
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Cross-Reactivity (Details)
- There is cross-reactivates with MMP-10 approximately 2 % and no detectable cross-reactivity with other MMPs.
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Sensitivity
- < 10 pg/mL
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Components
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96-well plate pre-coated with anti-MMP-3, Mouse
Lyophilized recombinant MMP-3, Mouse standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
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Plate
- Pre-coated
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Protocol
- Sandwich ELISA quantitative detection kit for Mouse MMP-3 in cell culture supernates, serum and plasma (heparin).Matrix metalloproteinase-3 (MMP-3) also called stromelysin or transin, is a proteoglycanase closely related to collagenase (MMP1) with a wide range of substrate specificities. The complete primary structure for human MMP-3, which has 477 residues including a 17-residue signal peptide. MMP-3 and collagenase are 54% identical in sequence, suggesting a common origin for the evolution of the two proteinases. MMP-3 and collagenase expression are coordinately modulated in synovial fibroblast cultures. MMP-3 is a secreted metalloprotease produced predominantly by connective tissue cells. Together with other metalloproteases, it can synergistically degrade the major components of the extracellular matrix. It is capable of degrading proteoglycan, fibronectin, laminin, and type IV collagen, but not interstitial type I collagen. MMP-3 genotype may be an important determinant of vascular remodeling and age-related arterial stiffening, with the heterozygote having the optimal balance between matrix accumulation and deposition.
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Restrictions
- For Research Use only
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Storage
- 4 °C/-20 °C
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- MMP3 (Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3))
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Alternative Name
- MMP-3
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Gene ID
- 17392
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UniProt
- P28862
Target See all MMP3 ELISA Kits
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