MMP7 ELISA Kit

Catalog No. ABIN2042607

The Human MMP7 ELISA Kit (ABIN2042607) is a Colorimetric ELISA Kit designed to quantify Human MMP7.

Quick Overview for MMP7 ELISA Kit (ABIN2042607)

Target: MMP7 (Matrix Metallopeptidase 7 (Matrilysin, Uterine) (MMP7))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 6-10000 pg/mL

Application: ELISA

Product Details MMP7 ELISA Kit

Minimum Detection Limit: 6 pg/mL

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 6 pg/mL

Components: 96-well plate pre-coated with anti-MMP-7, Human
Lyophilized recombinant MMP-7, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Application Details

Plate: Pre-coated

Protocol: Sandwich ELISA quantitative detection kit for Human MMP-7 in cell culture supernates, serum, plasma (heparin), saliva and urine.Matrix metalloproteinase-7 (MMP-7) previously called putative metalloproteinase I (PUMP1) or matrilysin. The PUMP1 gene has been identified through studies of collagenase-related connective-tissue-degrading metalloproteinases produced by human tumors. The PUMP I protein has 267 amino acids and is significantly shorter than stromelysin or collagenase (477 and 469 amino acids, respectively). Matrix metalloproteinases play a crucial role in tumor invasion and metastasis.1 Matrilysin, a member of the matrix metalloproteinase family, is structurally different from the other matrix metalloproteinases by virtue of the absence of a conserved COOH-terminal protein domain. In addition, matrilysin mRNA is regulated in a specific and distinct manner in normal and malignant tissues.2 Matrilysin has been shown to correlate with nodal or distant metastasis in colorectal carcinomas, however, its implication in early invasive colorectal carcinomas has not been determined.1 Matrilysin is also a mediator of pulmonary fibrosis and a potential therapeutic target.3 The standard product used in this kit is recombinant human MMP-7, consisting of 250 amino acids with the molecular mass of 28kD. The detected MMP-7 includes zymogen and active enzyme.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for MMP7

Target: MMP7 (Matrix Metallopeptidase 7 (Matrilysin, Uterine) (MMP7))

Alternative Name: MMP-7

Gene ID: 4316

UniProt: P09237

Pathways: Production of Molecular Mediator of Immune Response