NGFB ELISA Kit

Catalog No. ABIN2042619

This Human NGFB ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human NGFB.

Quick Overview for NGFB ELISA Kit (ABIN2042619)

Target: NGFB (Nerve Growth Factor beta (NGFB))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6-1000 pg/mL

Application: ELISA

Product Details NGFB ELISA Kit

Minimum Detection Limit: 15.6 pg/mL

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 1 pg/mL

Components: A: Microtiter Strip, 1x96 wells
B: NGF Standard, 2x10 ng
C: Anti-human NGF (Biotin), 1x130 µL

Application Details

Plate: Pre-coated

Protocol: NGF/NGF beta BioAssay-ELISA Kit, Human is a sandwich ELISA quantitative detection kit for human NGF/NGF beta in cell culture supernates and serum.Nerve growth factor (NGF) is a polypeptide involved in the regulation of growth and differentiation of sympathetic and certain sensory neurons. NGF is thought to have a profound effect on the development and maintenance of sympathetic and embryonic sensory neurones. NGF activity isolated from the male mouse submaxillary gland (MSG) consists of three types of subunits, alpha, beta and gamma, which specifically interact to form a 7S, approximately 130,000-molecular weight (Mr) complex. The 7S complex contains two identical 118-amino acid beta-chains, which are solely responsible for the nerve growth-stimulating activity of NGF (1). NGF, which is expressed by inflammatory cells and effects changes that lead to increased neural responsiveness, could be a pivotal mediator in allergic rhinitis (2). The standard product used in this kit is human 2.5S NGF, which is a dimmer linking with two polypeptide chains of 120 amino acids.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for NGFB

Target: NGFB (Nerve Growth Factor beta (NGFB))

Gene ID: 4803

UniProt: P01138

Pathways: NF-kappaB Signaling, RTK Signaling, Regulation of Cell Size