NGFB ELISA Kit
Quick Overview for NGFB ELISA Kit (ABIN2042620)
Target
See all NGFB ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
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Minimum Detection Limit
- 31.2 pg/mL
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Cross-Reactivity (Details)
- There is no detectable cross-reactivity with BDGF, GDNF, CNTF, NT3 and NT4.
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Sensitivity
- < 1 pg/mL
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Components
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96-well plate pre-coated with anti-NGF/NGF beta, Mouse
Lyophilized recombinant NGF/NGF beta, Mouse standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
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Nerve Growth Factor beta (NGFB) ELISA Kit
NGFB Reactivity: Human Colorimetric Sandwich ELISA 6.86 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Nerve Growth Factor beta (NGFB) ELISA KitNGFB Reactivity: Human Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Nerve Growth Factor beta (NGFB) ELISA KitNGFB Reactivity: Rat Colorimetric Sandwich ELISA 39.063 pg/mL - 2500 pg/mL Plasma, Serum, Tissue Homogenate
Nerve Growth Factor beta (NGFB) ELISA KitNGFB Reactivity: Rabbit Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Nerve Growth Factor beta (NGFB) ELISA KitNGFB Reactivity: Mouse Colorimetric Sandwich ELISA 1.56 pg/mL - 100 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Nerve Growth Factor beta (NGFB) ELISA KitNGFB Reactivity: Human Colorimetric Sandwich ELISA 16-2000 pg/mL Cell Lysate, Plasma, Serum
Nerve Growth Factor beta (NGFB) ELISA KitNGFB Reactivity: Human Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Nerve Growth Factor beta (NGFB) ELISA KitNGFB Reactivity: Rat Colorimetric Sandwich ELISA 15-15000 pg/mL Cell Lysate, Tissue Lysate
Nerve Growth Factor beta (NGFB) ELISA KitNGFB Reactivity: Rat Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Serum
Nerve Growth Factor beta (NGFB) ELISA KitNGFB Reactivity: Human Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Serum
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Plate
- Pre-coated
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Protocol
- Sandwich ELISA quantitative detection kit for Mouse NGF/NGF beta in cell culture supernates and serum.Nerve growth factor (NGF) is a polypeptide involved in the regulation of growth and differentiation of sympathetic and certain sensory neurons. NGF is thought to have a profound effect on the development and maintenance of sympathetic and embryonic sensory neurones. NGF activity isolated from the male mouse submaxillary gland (MSG) consists of three types of subunits, alpha, beta and gamma, which specifically interact to form a 7S, approximately 130,000-molecular weight (Mr) complex. The 7S complex contains two identical 118-amino acid beta-chains, which are solely responsible for the nerve growth-stimulating activity of NGF.1 NGF, which is expressed by inflammatory cells and effects changes that lead to increased neural responsiveness, could be a pivotal mediator in allergic rhinitis.2
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Restrictions
- For Research Use only
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Storage
- 4 °C/-20 °C
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- NGFB (Nerve Growth Factor beta (NGFB))
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Gene ID
- 18049
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UniProt
- P01139
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Pathways
- NF-kappaB Signaling, RTK Signaling, Regulation of Cell Size
Target See all NGFB ELISA Kits
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