NGFB ELISA Kit

Catalog No. ABIN2042621

This Colorimetric ELISA kit is designed for the quantitative measurement of Rat NGFB.

Quick Overview for NGFB ELISA Kit (ABIN2042621)

Target: NGFB (Nerve Growth Factor beta (NGFB))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6-1000 pg/mL

Application: ELISA

Product Details NGFB ELISA Kit

Minimum Detection Limit: 15.6 pg/mL

Cross-Reactivity (Details): There is no detectable cross-reactivity with BDNF, GDNF, CNTF, NT3, and NT4.

Sensitivity: < 1 pg/mL

Components: 96-well plate pre-coated with anti-NGF/NGF beta, Rat
Lyophilized recombinant NGF/NGF beta, Rat standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Application Details

Plate: Pre-coated

Protocol: Sandwich ELISA quantitative detection kit for Rat NGF/NGF beta in cell culture supernates and serum.Nerve growth factor (NGF) is a polypeptide involved in the regulation of growth and differentiation of sympathetic and certain sensory neurons. NGF is thought to have a profound effect on the development and maintenance of sympathetic and embryonic sensory neurones. NGF activity isolated from the male mouse submaxillary gland (MSG) consists of three types of subunits, alpha, beta and gamma, which specifically interact to form a 7S, approximately 130,000-molecular weight (Mr) complex. The 7S complex contains two identical 118-amino acid beta-chains, which are solely responsible for the nerve growth-stimulating activity of NGF.1 NGF, which is expressed by inflammatory cells and effects changes that lead to increased neural responsiveness, could be a pivotal mediator in allergic rhinitis.2

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for NGFB

Target: NGFB (Nerve Growth Factor beta (NGFB))

Gene ID: 310738

UniProt: P25427

Pathways: NF-kappaB Signaling, RTK Signaling, Regulation of Cell Size