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PLAT ELISA Kit

This Human PLAT ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human PLAT.
Catalog No. ABIN2042637

Quick Overview for PLAT ELISA Kit (ABIN2042637)

Target

See all PLAT ELISA Kits
PLAT (Plasminogen Activator, Tissue (PLAT))

Reactivity

  • 8
  • 8
  • 4
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
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  • 1
Human

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

31.2-2000 pg/mL

Application

ELISA
  • Minimum Detection Limit

    31.2 pg/mL

    Cross-Reactivity (Details)

    There is no detectable cross-reactivity with other relevant proteins.

    Sensitivity

    < 10 pg/mL

    Components

    96-well plate pre-coated with anti-PLAT/TPA, Human
    Lyophilized recombinant PLAT/TPA, Human standard, 10 ng/tube, 2Ea
    Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
    Sample diluent buffer, 30 mL
    Antibody diluent buffer, 10 mL
    ABC diluent buffer, 10 mL
    TMB color developing agent, 10 mL
    TMB stop solution, 10 mL
  • Plate

    Pre-coated

    Protocol

    Sandwich ELISA quantitative detection kit for Human PLAT/TPA in cell culture supernates, serum, plasma (heparin, EDTA, citrate) and urine.Plasminogen activator, tissue, also called PLAT or TPA is a serine protease. This gene encodes tissue-type plasminogen activator, a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Tissue-type plasminogen activator is synthesized as a single chain which is cleaved by plasmin to a two chain disulfide linked protein. This gene was mapped to 8p11.21. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding, decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism.

    Restrictions

    For Research Use only
  • Storage

    4 °C/-20 °C
  • Target See all PLAT ELISA Kits

    PLAT (Plasminogen Activator, Tissue (PLAT))

    Alternative Name

    PLAT/TPA

    Gene ID

    5327

    UniProt

    P00750

    Pathways

    Autophagy, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling, SARS-CoV-2 Protein Interactome
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