Soluble E-Selectin ELISA Kit

Catalog No. ABIN2042658

The Human Soluble E-Selectin ELISA Kit (ABIN2042658) is a Colorimetric ELISA Kit designed to quantify Human Soluble E-Selectin.

Quick Overview for Soluble E-Selectin ELISA Kit (ABIN2042658)

Target: Soluble E-Selectin (sE-Selectin) (Selectin E (Soluble) (sE-Selectin))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 5-8000 pg/mL

Application: ELISA

Product Details Soluble E-Selectin ELISA Kit

Minimum Detection Limit: 5 pg/mL

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 4 pg/mL

Components: 96-well plate pre-coated with anti-sE-Selectin, Human
Lyophilized recombinant sE-Selectin, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Application Details

Plate: Pre-coated

Protocol: Sandwich ELISA quantitative detection kit for Human E-Selectin in cell culture supernates, serum and plasma (heparin, citrate).E-selectin, also called endothelial-leukocyte adhesion molecule-1 (ELAM-1), is a cell surface glycoprotein expressed by cytokine-activated endothelium that mediates the adhesion of blood neutrophils.1 An increased expression of E-selectin has been observed in the arterial endothelium interacting with lymphocytes and macrophages in human atherosclerotic lesions.2 E-selectin plays a critical role in mediating tissue-specific homing of T cells into skin, and of primitive hematopoietic progenitor cells (HPCs) into bone marrow (BM).3 A structurally and functionally related group of genes, lymph node homing receptor (LHR), granule membrane protein 140 (GMP-140), and endothelial leukocyte adhesion molecule 1 (ELAM-1) are shown to constitute a gene cluster on mouse and human chromosome 1.4 The standard product used in this kit is recombinant human E-Selectin, excluding intercellular E-Selectin and transmembrane domain. It consists of totally 535 amino acids with the molecular mass of 58.6kD.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for Soluble E-Selectin

Target: Soluble E-Selectin (sE-Selectin) (Selectin E (Soluble) (sE-Selectin))

Alternative Name: sE-Selectin

Gene ID: 6401

UniProt: P16581