sFAS/Apo-1 ELISA Kit

Catalog No. ABIN2042662

Human sFAS/Apo-1 ELISA Kit, Colorimetric assay for quantification of Human sFAS/Apo-1.

Quick Overview for sFAS/Apo-1 ELISA Kit (ABIN2042662)

Target: sFAS/Apo-1 (sFAS) (Soluble Factor-Related Apoptosis (sFAS))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2-2000 pg/mL

Application: ELISA

Product Details sFAS/Apo-1 ELISA Kit

Minimum Detection Limit: 31.2 pg/mL

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 3 pg/mL

Components: 96-well plate pre-coated with anti-sFAS, Human
Lyophilized recombinant sFAS, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Application Details

Plate: Pre-coated

Protocol: Sandwich ELISA quantitative detection kit for Human Soluble FAS in cell culture supernates, serum and plasma (heparin, EDTA, citrate).Fas, also known as APO-1, CD95 and TNFRSF6, is a member of the nerve growth factor (NGF)/tumour necrosis factor (TNF) receptor superfamily and mediates apoptosis.1 The nucleotide sequence of the cDNAs reveales that the molecule coding for the Fas antigen determinant is a 319 amino acid polypeptide with a single transmembrane domain. The extracellular domain is rich in cysteine residue, and shows a similarity to that of human tumor necrosis factor receptors, human nerve growth factor receptor, and human B cell antigen CD40.2 The APO-1 antigen as defined by the mouse monoclonal antibody anti-APO-1 is previously found to be expressed on the cell surface of activated human T and B lymphocytes and a variety of malignant human lymphoid cell lines. The APO-1 antigen is found to be a membrane glycoprotein of 48kD.3 Fas antigen is expressed and functional on papillary thyroid cancer cells and this may have potential therapeutic significance.4 Fas can play a role as an inducer of both neurite growth in vitro and accelerates recovery after nerve injury in vivo.5 The FAS and FASL triggered apoptosis pathway plays an important role in human carcinogenesis.6

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for sFAS/Apo-1

Target: sFAS/Apo-1 (sFAS) (Soluble Factor-Related Apoptosis (sFAS))

Gene ID: 355

UniProt: P25445