Survivin ELISA Kit

Catalog No. ABIN2042670

This Human Survivin ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human Survivin.

Quick Overview for Survivin ELISA Kit (ABIN2042670)

Target: Survivin (BIRC5) (Baculoviral IAP Repeat-Containing 5 (BIRC5))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 62.5-4000 pg/mL

Application: ELISA

Product Details Survivin ELISA Kit

Minimum Detection Limit: 62.5 pg/mL

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 2 pg/mL

Components: 96-well plate pre-coated with anti-Survivin, Human
Lyophilized recombinant Survivin, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Application Details

Plate: Pre-coated

Protocol: Sandwich ELISA quantitative detection kit for Human Survivin in cell culture supernates, serum and plasma (heparin, EDTA).Survivin (also termed API4) belongs to the family of genes known as inhibitors of apoptosis, and it has been implicated in both prevention of cell death and control of mitosis. Survivin is prominently expressed in transformed cell lines and in all the most common human cancers of lung, colon, pancreas, prostate and breast in vivo. It is also found in approximately 50% of high-grade non-Hodgkin's lymphomas. Survivin encodes a deduced 142-amino acid protein and localizes on chromosome 17q25. The overexpression of it in cancer may overcome an apoptotic checkpoint and favour aberrant progression of transformed cells through mitosis. The standard product used in this kit is recombinant human Survivin with the molecular mass of 34kD.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for Survivin

Target: Survivin (BIRC5) (Baculoviral IAP Repeat-Containing 5 (BIRC5))

Gene ID: 332

UniProt: O15392

Pathways: Apoptosis, Cell Division Cycle, Nuclear Hormone Receptor Binding