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TEK ELISA Kit

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse TEK.
Catalog No. ABIN2042672

Quick Overview for TEK ELISA Kit (ABIN2042672)

Target

See all TEK ELISA Kits
TEK (TEK Tyrosine Kinase, Endothelial (TEK))

Reactivity

  • 12
  • 9
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Mouse

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

5-8000 pg/mL

Application

ELISA
  • Minimum Detection Limit

    5 pg/mL

    Cross-Reactivity (Details)

    There is no detectable cross-reactivity with other relevant proteins.

    Sensitivity

    < 10 pg/mL

    Components

    96-well plate pre-coated with anti-TEK/TIE2, Mouse
    Lyophilized recombinant TEK/TIE2, Mouse standard, 10 ng/tube, 2Ea
    Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
    Sample diluent buffer, 30 mL
    Antibody diluent buffer, 10 mL
    ABC diluent buffer, 10 mL
    TMB color developing agent, 10 mL
    TMB stop solution, 10 mL
  • Plate

    Pre-coated

    Protocol

    Sandwich ELISA quantitative detection kit for Mouse TEK/TIE2 in cell culture supernates, tissue homogenates, serum and plasma (heparin, EDTA).Angiopoietin-1 receptor, also called TEK or TIE2 is a protein that in humans is encoded by the TEK gene. This gene is mapped to 9p21.2. The TEK receptor tyrosine kinase is expressed almost exclusively in endothelial cells in mice, rats, and humans. This receptor possesses a unique extracellular domain containing 2 immunoglobulin-like loops separated by 3 epidermal growth factor-like repeats that are connected to 3 fibronectin type III-like repeats. The ligand for the receptor is angiopoietin-1. Defects in TEK are associated with inherited venous malformations, the TEK signaling pathway appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis. TEK is closely related to the TIE receptor tyrosine kinase.

    Restrictions

    For Research Use only
  • Storage

    4 °C/-20 °C
  • Target See all TEK ELISA Kits

    TEK (TEK Tyrosine Kinase, Endothelial (TEK))

    Alternative Name

    TEK/TIE2

    Gene ID

    21687

    UniProt

    Q02858

    Pathways

    RTK Signaling, Growth Factor Binding
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