TEK ELISA Kit
Quick Overview for TEK ELISA Kit (ABIN2042679)
Target
See all TEK ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
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Minimum Detection Limit
- 6 pg/mL
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Cross-Reactivity (Details)
- There is cross-reactivates with human TIE1< 0.1 % .
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Sensitivity
- < 5 pg/mL
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Components
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96-well plate pre-coated with anti-TIE2, Human
Lyophilized recombinant TIE2, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
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TEK Tyrosine Kinase, Endothelial (TEK) ELISA Kit
TEK Reactivity: Human Colorimetric Sandwich ELISA 0.94 ng/mL - 60 ng/mL Plasma, Serum, Tissue Homogenate
TEK Tyrosine Kinase, Endothelial (TEK) ELISA KitTEK Reactivity: Rat Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
TEK Tyrosine Kinase, Endothelial (TEK) ELISA KitTEK Reactivity: Mouse Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
TEK Tyrosine Kinase, Endothelial (TEK) ELISA KitTEK Reactivity: Mouse AA 23-744 Colorimetric Sandwich ELISA 125-8000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum, Tissue Homogenate
TEK Tyrosine Kinase, Endothelial (TEK) ELISA KitTEK Reactivity: Human AA 23-745 Colorimetric Sandwich ELISA 156-10000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
TEK Tyrosine Kinase, Endothelial (TEK) ELISA KitTEK Reactivity: Human Colorimetric Sandwich ELISA 157-10000 pg/mL Cell Lysate, Plasma, Serum
TEK Tyrosine Kinase, Endothelial (TEK) ELISA KitTEK Reactivity: Mouse Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Tissue Homogenate
TEK Tyrosine Kinase, Endothelial (TEK) ELISA KitTEK Reactivity: Human Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
TEK Tyrosine Kinase, Endothelial (TEK) ELISA KitTEK Reactivity: Human Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Culture Supernatant, Plasma, Serum
TEK Tyrosine Kinase, Endothelial (TEK) ELISA KitTEK Reactivity: Mouse Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
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Plate
- Pre-coated
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Protocol
- Sandwich ELISA quantitative detection kit for Human TIE2 in cell culture supernates, serum and plasma (heparin, EDTA).Tyrosine kinase with Ig and EGF homology domain 2 (Tie-2), also called TEK tyrosine kinase, endothelial (TEK). Tie-2 and tie-1 are expressed in early embryonic vascular system and in maternal decidual vascular endothelial cells, where the vasculature undergoes an active angiogenesis. Tie-2, but not tie-1, expression was also detected in extraembryonic mesoderm of the amnion. Angiogenesis is coordinated with follicular cell growth in goitrogenesis. The angiopoietins, Ang-1 and Ang-2, are angiogenic growth factors acting through Tie-2. Tie-2 and Ang-1 are expressed in thyroid epithelial and endothelial cells, and Tie-2 is regulated by TSH and cAMP in follicular cells. And Tie-2 expression is increased in goiter in both humans and rats, consistent with a role in goitrogenesis. Tie2/Ang-1 signaling pathway plays a critical role in the maintenance of HSCs in a quiescent state in the BM niche. And the Tie-2 signaling pathway is also critical for endothelial cell-smooth muscle cell communication in venous morphogenesis.
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Restrictions
- For Research Use only
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Storage
- 4 °C/-20 °C
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- TEK (TEK Tyrosine Kinase, Endothelial (TEK))
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Alternative Name
- TIE2
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Gene ID
- 7010
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UniProt
- Q02763
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Pathways
- RTK Signaling, Growth Factor Binding
Target See all TEK ELISA Kits
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