TEK ELISA Kit

Catalog No. ABIN2042679

This Human TEK ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human TEK.

Quick Overview for TEK ELISA Kit (ABIN2042679)

Target: TEK (TEK Tyrosine Kinase, Endothelial (TEK))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 6-10000 pg/mL

Application: ELISA

Product Details TEK ELISA Kit

Minimum Detection Limit: 6 pg/mL

Cross-Reactivity (Details): There is cross-reactivates with human TIE1< 0.1 % .

Sensitivity: < 5 pg/mL

Components: 96-well plate pre-coated with anti-TIE2, Human
Lyophilized recombinant TIE2, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Application Details

Plate: Pre-coated

Protocol: Sandwich ELISA quantitative detection kit for Human TIE2 in cell culture supernates, serum and plasma (heparin, EDTA).Tyrosine kinase with Ig and EGF homology domain 2 (Tie-2), also called TEK tyrosine kinase, endothelial (TEK). Tie-2 and tie-1 are expressed in early embryonic vascular system and in maternal decidual vascular endothelial cells, where the vasculature undergoes an active angiogenesis. Tie-2, but not tie-1, expression was also detected in extraembryonic mesoderm of the amnion. Angiogenesis is coordinated with follicular cell growth in goitrogenesis. The angiopoietins, Ang-1 and Ang-2, are angiogenic growth factors acting through Tie-2. Tie-2 and Ang-1 are expressed in thyroid epithelial and endothelial cells, and Tie-2 is regulated by TSH and cAMP in follicular cells. And Tie-2 expression is increased in goiter in both humans and rats, consistent with a role in goitrogenesis. Tie2/Ang-1 signaling pathway plays a critical role in the maintenance of HSCs in a quiescent state in the BM niche. And the Tie-2 signaling pathway is also critical for endothelial cell-smooth muscle cell communication in venous morphogenesis.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for TEK

Target: TEK (TEK Tyrosine Kinase, Endothelial (TEK))

Alternative Name: TIE2

Gene ID: 7010

UniProt: Q02763

Pathways: RTK Signaling, Growth Factor Binding