Thrombopoietin ELISA Kit

Catalog No. ABIN2042708

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse Thrombopoietin.

Quick Overview for Thrombopoietin ELISA Kit (ABIN2042708)

Target: Thrombopoietin (THPO)

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 31.2-2000 pg/mL

Application: ELISA

Product Details Thrombopoietin ELISA Kit

Minimum Detection Limit: 31.2 pg/mL

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: < 5 pg/mL

Components: 96-well plate pre-coated with anti-TPO, Mouse
Lyophilized recombinant TPO, Mouse standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL

Application Details

Plate: Pre-coated

Protocol: Sandwich ELISA quantitative detection kit for Mouse TPO in cell culture supernates, serum and plasma (heparin, EDTA).Thrombopoietin (TPO) is also called megakaryocyte growth and development factor (MGDF). TPO, a lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from committed progenitor cells, is believed to be the major physiological regulator of circulating platelet levels.1 Human, dog, and mouse cDNAs for MGDF are highly conserved and encode open reading frames for proteins of 353, 352, and 356 amino acids, respectively, including predicted signal peptides. MGDF is a novel cytokine that regulates megakaryocyte development and is a ligand for the MPL receptor. The human MGDF gene has been mapped to chromosome 3q26.3. 2,3 The standard product used in this kit is gene recombinant mouse TPO, consisting of 335 amino acids with the molecular mass of 35kD. As a result of glycosylation, the molecular mass is 75kD.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for Thrombopoietin

Target: Thrombopoietin (THPO)

Alternative Name: Thrombopoietin (TPO)

Gene ID: 21832

UniProt: P40226

Pathways: JAK-STAT Signaling, Hormone Activity