PLAU ELISA Kit
Quick Overview for PLAU ELISA Kit (ABIN2042715)
Target
See all PLAU ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
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Minimum Detection Limit
- 62.5 pg/mL
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Cross-Reactivity (Details)
- There is no detectable cross-reactivity with other relevant proteins.
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Sensitivity
- < 5 pg/mL
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Components
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96-well plate pre-coated with anti-uPA/PLAU, Human
Lyophilized recombinant uPA/PLAU, Human standard, 10 ng/tube, 2Ea
Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
Sample diluent buffer, 30 mL
Antibody diluent buffer, 10 mL
ABC diluent buffer, 10 mL
TMB color developing agent, 10 mL
TMB stop solution, 10 mL
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Plasminogen Activator, Urokinase (PLAU) ELISA Kit
PLAU Reactivity: Human Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Tissue Homogenate
Plasminogen Activator, Urokinase (PLAU) ELISA KitPLAU Reactivity: Mouse Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum
Plasminogen Activator, Urokinase (PLAU) ELISA KitPLAU Reactivity: Mouse Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Plasminogen Activator, Urokinase (PLAU) ELISA KitPLAU Reactivity: Monkey Colorimetric Sandwich ELISA 78.125 pg/mL - 5000 pg/mL Plasma, Serum, Tissue Homogenate
Plasminogen Activator, Urokinase (PLAU) ELISA KitPLAU Reactivity: Horse Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum, Tissue Homogenate
Plasminogen Activator, Urokinase (PLAU) ELISA KitPLAU Reactivity: Rabbit Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Plasminogen Activator, Urokinase (PLAU) ELISA KitPLAU Reactivity: Rat Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Plasminogen Activator, Urokinase (PLAU) ELISA KitPLAU Reactivity: Cow Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum
Plasminogen Activator, Urokinase (PLAU) ELISA KitPLAU Reactivity: Pig Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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Plate
- Pre-coated
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Protocol
- Sandwich ELISA quantitative detection kit for Human uPA/PLAU in cell culture supernates, serum and plasma (heparin, EDTA).Plasminogen activator,urokinase (PLAU, uPA) converts plasminogen to plasmin. Plasmin is involved in processing of amyloid precursor protein and degrades secreted and aggregated amyloid-beta, a hallmark of Alzheimer disease (AD). Urokinase has a molecular mass of about 54kD and is composed of 2 disulfide-linked chains, A and B, of molecular masses 18kD and 33kD, respectively. It localized on 10q24. uPA facilitates cell migration by localizing proteolisys on the cell surface and by inducing intracellular signalling pathways. In human vascular smooth muscle cell (VSMC), uPA stimulates migration via the uPA receptor (uPAR) signalling complex containing TYK2 and phosphatidylinositol 3-kinase (PI3-K).
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Restrictions
- For Research Use only
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Storage
- 4 °C/-20 °C
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- PLAU (Plasminogen Activator, Urokinase (PLAU))
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Alternative Name
- uPA/PLAU
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Gene ID
- 5328
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UniProt
- P00749
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Pathways
- Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy, Smooth Muscle Cell Migration
Target See all PLAU ELISA Kits
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