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VEGFC ELISA Kit

Human VEGFC ELISA Kit Colorimetric assay for quantification of Human VEGFC.
Catalog No. ABIN2042722

Quick Overview for VEGFC ELISA Kit (ABIN2042722)

Target

See all VEGFC ELISA Kits
VEGFC (Vascular Endothelial Growth Factor C (VEGFC))

Reactivity

  • 9
  • 4
  • 3
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Human

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

62.5-4000 pg/mL

Application

ELISA
  • Minimum Detection Limit

    62.5 pg/mL

    Cross-Reactivity (Details)

    There is no detectable cross-reactivity with other relevant proteins.

    Sensitivity

    < 3 pg/mL

    Components

    96-well plate pre-coated with anti-VEGF-C, Human
    Lyophilized recombinant VEGF-C, Human standard, 10 ng/tube, 2Ea
    Avidin-Biotin-Peroxidase Complex (ABC), 130 µL (1:100 dilution)
    Sample diluent buffer, 30 mL
    Antibody diluent buffer, 10 mL
    ABC diluent buffer, 10 mL
    TMB color developing agent, 10 mL
    TMB stop solution, 10 mL
  • Plate

    Pre-coated

    Protocol

    Sandwich ELISA quantitative detection kit for Human VEGF-C in cell culture supernates, serum and plasma (heparin, EDTA).Vascular endothelial growth factor C is a VEGF. The human gene encoding it is VEGFC. The protein encoded by this gene is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family, is active in angiogenesis, lymphangiogenesis and endothelial cell growth and survival, and can also affect the permeability of blood vessels. This secreted protein undergoes a complex proteolytic maturation, generating multiple processed forms which bind and activate VEGFR-3 receptors. Only the fully processed form can bind and activate VEGFR-2 receptors. This protein is structurally and functionally similar to vascular endothelial growth factor D (VEGF-D). The C terminus of VEGFC has cysteine-rich repeat units characteristic of the Balbiani ring 3 protein (BR3P) of the midge Chironomus tentans.1,2 The standard product used in this kit is recombinant human VEGF-C, consisting of 135 amino acids with the molecular mass of 23kD after glycosylation.

    Restrictions

    For Research Use only
  • Storage

    4 °C/-20 °C
  • Target See all VEGFC ELISA Kits

    VEGFC (Vascular Endothelial Growth Factor C (VEGFC))

    Alternative Name

    VEGF-C

    Gene ID

    7424

    UniProt

    P49767

    Pathways

    RTK Signaling, Signaling Events mediated by VEGFR1 and VEGFR2
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