HSD11B1L ELISA Kit

Catalog No. ABIN2042731

Human HSD11B1L ELISA Kit, Colorimetric assay for quantification of Human HSD11B1L.

Quick Overview for HSD11B1L ELISA Kit (ABIN2042731)

Target: HSD11B1L (Hydroxysteroid (11-Beta) Dehydrogenase 1-Like (HSD11B1L))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details HSD11B1L ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.065 ng/mL

Application Details

Plate: Pre-coated

Protocol: 11-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD11b3) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 11-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD11b3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to 11-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD11b3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain 11-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD11b3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of 11-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD11b3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for HSD11B1L

Target: HSD11B1L (Hydroxysteroid (11-Beta) Dehydrogenase 1-Like (HSD11B1L))

Alternative Name: 11-beta-Hydroxysteroid Dehydrogenase Type 3 (HSD11b3)