ADAM33 ELISA Kit

Catalog No. ABIN2042743

Human ADAM33 ELISA Kit, Colorimetric assay for quantification of Human ADAM33.

Quick Overview for ADAM33 ELISA Kit (ABIN2042743)

Target: ADAM33 (ADAM Metallopeptidase Domain 33 (ADAM33))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details ADAM33 ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.060 ng/mL

Application Details

Plate: Pre-coated

Protocol: A Disintegrin And Metalloprotease 33 (ADAM33) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to A Disintegrin And Metalloprotease 33 (ADAM33). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to A Disintegrin And Metalloprotease 33 (ADAM33). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain A Disintegrin And Metalloprotease 33 (ADAM33), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of A Disintegrin And Metalloprotease 33 (ADAM33) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for ADAM33

Target: ADAM33 (ADAM Metallopeptidase Domain 33 (ADAM33))

Alternative Name: A Disintegrin And Metalloprotease 33 (ADAM33)