ACAT1 ELISA Kit

Catalog No. ABIN2042749

Mouse ACAT1 ELISA Kit Colorimetric assay for quantification of Mouse ACAT1.

Quick Overview for ACAT1 ELISA Kit (ABIN2042749)

Target: ACAT1 (Acetyl-CoA Acetyltransferase 1 (ACAT1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details ACAT1 ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.052 ng/mL

Application Details

Plate: Pre-coated

Protocol: Acetyl Coenzyme A Acetyltransferase 1 (ACAT1) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Acetyl Coenzyme A Acetyltransferase 1 (ACAT1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Acetyl Coenzyme A Acetyltransferase 1 (ACAT1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Acetyl Coenzyme A Acetyltransferase 1 (ACAT1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Acetyl Coenzyme A Acetyltransferase 1 (ACAT1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for ACAT1

Target: ACAT1 (Acetyl-CoA Acetyltransferase 1 (ACAT1))

Alternative Name: Acetyl Coenzyme A Acetyltransferase 1 (ACAT1)