Anion/Sugar Transporter ELISA Kit

Catalog No. ABIN2042875

Human Anion/Sugar Transporter ELISA Kit, Colorimetric assay for quantification of Human Anion/Sugar Transporter.

Quick Overview for Anion/Sugar Transporter ELISA Kit (ABIN2042875)

Target: Anion/Sugar Transporter (AST)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details Anion/Sugar Transporter ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Sensitivity: < 0.112 ng/mL

Application Details

Plate: Pre-coated

Protocol: Anion/Sugar Transporter (AST) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Anion/Sugar Transporter (AST). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Anion/Sugar Transporter (AST). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Anion/Sugar Transporter (AST), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Anion/Sugar Transporter (AST) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for Anion/Sugar Transporter

Target: Anion/Sugar Transporter (AST)