GAD Ab ELISA Kit

Catalog No. ABIN2042887

Human GAD Ab ELISA Kit, Colorimetric assay for quantification of Human GAD Ab.

Quick Overview for GAD Ab ELISA Kit (ABIN2042887)

Target: GAD Ab (Glutamic Acid Decarboxylase Antibody (GAD Ab))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 1.56-100 ng/mL

Application: ELISA

Product Details GAD Ab ELISA Kit

Minimum Detection Limit: 1.56 ng/mL

Analytical Method: Quantitative

Sensitivity: < 0.65 ng/mL

Components: A: Microtiter Plate, 96 wells, Pre-coated, ready to use
B: Standard, 2x1vial
C: Standard Diluent, 1x20 mL
D: Detection Reagent A, 1x120 µL
E: Assay Diluent A, 1x10 mL
F: TMB Substrate, 1x0 mL
G: Stop Solution, 1x0 mL
H: Wash Buffer, 30X, 1x20 mL

Application Details

Plate: Pre-coated

Protocol: The Human Anti-Glutamic Acid Decarboxylase Antibodies (Anti-GAD) ELISA Kit is an enzyme immunoassay for the in vitro quantitative measurement of Anti-GAD in human serum, plasma and other biological fluids.The microtiter plate has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells and the liquid in each well containing unbound material is aspirated after the required incubation period. HRP-conjugated secondary antibody is then added. After incubation the unbound conjugate is washed off. After TMB substrate solution is added, those wells that contain Anti-GAD will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-GAD in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for GAD Ab

Target: GAD Ab (Glutamic Acid Decarboxylase Antibody (GAD Ab))

Alternative Name: Anti-Glutamic Acid Decarboxylase Antibodies (Anti-GAD)

Target Type: Antibody, Antibody