Anti-Insulin Receptor Antibody ELISA Kit
Quick Overview for Anti-Insulin Receptor Antibody ELISA Kit (ABIN2042895)
Target
Reactivity
Detection Method
Method Type
Detection Range
Application
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Minimum Detection Limit
- 3.12 ng/mL
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Sensitivity
- < 1.29 ng/mL
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Plate
- Pre-coated
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Protocol
- Anti-Insulin Receptor Antibody (AIRA) BioAssay-ELISA Kit (Human) is based on Sandwich ELISA. The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Anti-Insulin Receptor Antibody (AIRA) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Anti-Insulin Receptor Antibody (AIRA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Restrictions
- For Research Use only
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Storage
- 4 °C/-20 °C
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- Anti-Insulin Receptor Antibody
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Alternative Name
- Anti-Insulin Receptor Antibody (AIRA)
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Target Type
- Antibody
Target
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