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APOA1 ELISA Kit

Dog APOA1 ELISA Kit, Colorimetric assay for quantification of Dog APOA1.
Catalog No. ABIN2042917
$1,214.07
Plus shipping costs $50.00
96 tests
Shipping to: United States
Delivery in 16 to 22 Business Days

Quick Overview for APOA1 ELISA Kit (ABIN2042917)

Target

See all APOA1 ELISA Kits
APOA1 (Apolipoprotein A-I (APOA1))

Reactivity

  • 17
  • 8
  • 7
  • 5
  • 4
  • 4
  • 4
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
Dog

Detection Method

Colorimetric

Method Type

Sandwich ELISA

Detection Range

31.25-2000 ng/mL

Application

ELISA
  • Minimum Detection Limit

    31.25 ng/mL

    Sensitivity

    < 14.8 ng/mL
  • Plate

    Pre-coated

    Protocol

    Apolipoprotein A1 (APOA1) BioAssay-ELISA Kit (Canine) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apolipoprotein A1 (APOA1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Apolipoprotein A1 (APOA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apolipoprotein A1 (APOA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Apolipoprotein A1 (APOA1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

    Restrictions

    For Research Use only
  • Storage

    4 °C/-20 °C
  • Target See all APOA1 ELISA Kits

    APOA1 (Apolipoprotein A-I (APOA1))

    Alternative Name

    Apolipoprotein A1 (APOA1)

    Pathways

    Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response, Lipid Metabolism
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