APOA1 ELISA Kit
Quick Overview for APOA1 ELISA Kit (ABIN2042917)
Target
See all APOA1 ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
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Minimum Detection Limit
- 31.25 ng/mL
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Sensitivity
- < 14.8 ng/mL
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Plate
- Pre-coated
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Protocol
- Apolipoprotein A1 (APOA1) BioAssay-ELISA Kit (Canine) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apolipoprotein A1 (APOA1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Apolipoprotein A1 (APOA1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apolipoprotein A1 (APOA1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Apolipoprotein A1 (APOA1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Restrictions
- For Research Use only
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Storage
- 4 °C/-20 °C
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- APOA1 (Apolipoprotein A-I (APOA1))
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Alternative Name
- Apolipoprotein A1 (APOA1)
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Pathways
- Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response, Lipid Metabolism
Target See all APOA1 ELISA Kits
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