APOH ELISA Kit

Catalog No. ABIN2042929

Mouse APOH ELISA Kit, Colorimetric assay for quantification of Mouse APOH.

Quick Overview for APOH ELISA Kit (ABIN2042929)

Target: APOH (Apolipoprotein H (Beta-2-Glycoprotein I) (APOH))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 3.12-200 ng/mL

Application: ELISA

Product Details APOH ELISA Kit

Minimum Detection Limit: 3.12 ng/mL

Sensitivity: < 1.42 ng/mL

Application Details

Plate: Pre-coated

Protocol: Apolipoprotein H (APOH) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Apolipoprotein H (APOH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Apolipoprotein H (APOH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Apolipoprotein H (APOH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Apolipoprotein H (APOH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for APOH

Target: APOH (Apolipoprotein H (Beta-2-Glycoprotein I) (APOH))

Alternative Name: Apolipoprotein H (APOH)