APOH ELISA Kit

Catalog No. ABIN4881879

Human APOH ELISA Kit, Colorimetric assay for quantification of Human APOH.

Quick Overview for APOH ELISA Kit (ABIN4881879)

Target: APOH (Apolipoprotein H (Beta-2-Glycoprotein I) (APOH))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 30 pg/mL - 6000 pg/mL

Application: ELISA

Sample Type: Plasma, Cell Culture Supernatant, Serum

Product Details APOH ELISA Kit

Minimum Detection Limit: 30 pg/mL

Purpose: Human ApoH ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.

Analytical Method: Quantitative

Specificity: Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human A2M, Adiponectin, Adipsin, Albumin, Angiogenin, ApoA1, ApoC1, ApoC2, ApoC3, ApoE, B2M, CD14, Clusterin, CRP, Cystatin C, ECM-1, Fetuin A, Lipocalin-2, LYVE-1, MBL, NAP-2, PF4, RBP4, SAA, Serpin A4, TfR, TSP-1, Transferrin, Vitronectin, vWF

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

,

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Suggested Plasma Dilution: 1,000,000 fold

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: May be stored for up to 6 months at 2° to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week.
Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.

Expiry Date: 6 months

Target Details for APOH

Target: APOH (Apolipoprotein H (Beta-2-Glycoprotein I) (APOH))

Alternative Name: ApoH

Background:

Gene Symbols: APOH, B2G1

Protein Name (Synonyms): Beta-2-glycoprotein 1 (APC inhibitor) (Activated protein C-binding protein) (Anticardiolipin cofactor) (Apolipoprotein H) (Apo-H) (Beta-2-glycoprotein I) (B2GPI) (Beta(2)GPI)

Gene ID: 350

UniProt: P02749