Aurora Kinase B ELISA Kit

Catalog No. ABIN2042979

This Human Aurora Kinase B ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human Aurora Kinase B.

Quick Overview for Aurora Kinase B ELISA Kit (ABIN2042979)

Target: Aurora Kinase B (AURKB)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details Aurora Kinase B ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.055 ng/mL

Application Details

Plate: Pre-coated

Protocol: Aurora Kinase B (AURKB) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aurora Kinase B (AURKB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aurora Kinase B (AURKB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aurora Kinase B (AURKB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Aurora Kinase B (AURKB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for Aurora Kinase B

Target: Aurora Kinase B (AURKB)

Pathways: TCR Signaling, Cell Division Cycle, Maintenance of Protein Location, Hepatitis C, Toll-Like Receptors Cascades