BHLHA15 ELISA Kit

Catalog No. ABIN2042997

The Human BHLHA15 ELISA Kit (ABIN2042997) is a Colorimetric ELISA Kit designed to quantify Human BHLHA15.

Quick Overview for BHLHA15 ELISA Kit (ABIN2042997)

Target: BHLHA15 (Basic Helix-Loop-Helix Family, Member A15 (BHLHA15))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details BHLHA15 ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.071 ng/mL

Application Details

Plate: Pre-coated

Protocol: Basic Helix Loop Helix Domain Containing Protein B8 (BHLHB8) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Basic Helix Loop Helix Domain Containing Protein B8 (BHLHB8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Basic Helix Loop Helix Domain Containing Protein B8 (BHLHB8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Basic Helix Loop Helix Domain Containing Protein B8 (BHLHB8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Basic Helix Loop Helix Domain Containing Protein B8 (BHLHB8) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for BHLHA15

Target: BHLHA15 (Basic Helix-Loop-Helix Family, Member A15 (BHLHA15))

Alternative Name: Basic Helix Loop Helix Domain Containing Protein B8 (BHLHB8)